Gene delivery vehicles

Poly (ethylenimine) (PEI) has been demonstrated as an efficient gene delivery vehicle both in vitro and in vivo (161-163). Linear (22 kDa) and branched PEI formulations of varying molecular weights (0.6-800 kDa) have been reported. While polyplexes from higher molecular weight branched PEIs (70-800 kDa) were found to be more efficient in vitro but on intravenous administration the smaller and linear PEIs seem in general to be more efficient (171). However, questions as to the... [Pg.353]

Ozbas-Turan, S., Aral, C., Kabasakal, L., Keyer-Uysal, M., Akbuga, J. (2003). Coencapsulation of two plasmids in chitosan microspheres as a non-viral gene delivery vehicle. J. Pharm. Pharm. Sci., 6(1), 27-32. [Pg.374]

Ding, Z.M., Cristiano, R.J., Roth, J.A., Takacs, B., Kuo, M.T. (1995). Malarial circumsporozoite protein is a novel gene delivery vehicle to primary hepatocyte cultures and cultured cells. J. Biol. Chem., 270, 3667-3676. [Pg.376]

Benns, J.M., Choi, J.S., Mahato, R.I., Park, J.S. and Kim, S.W. (2000) pH-sensitive cationic polymer gene delivery vehicle V-Ac-poly(l- histidine)-graft-poly(l- lysine) comb shaped polymer. Bioconjug. Chem., 11, 637-645. [Pg.166]

Godbey, W.T., Wu, K.K. and Mikos, A.G. (1999a) Size matters Molecular weight affects the efficiency of poly (ethylenimine) as a gene delivery vehicle. J. Biomed. Mater. Res., 45, 268-275. [Pg.353]

Carter PJ, Samulski RJ (2000) Adeno-associated viral vectors as gene delivery vehicles. Int J Mol Med 6(1) 17-27... [Pg.12]

As previously discussed, the protection of pDNA against degrading enzymes is a critical parameter for a non-viral carrier. Such ability is needed for the polyplex to protect the nucleic acid for an extended period of time in the blood while the polyplex circulates and distributes. Research conducted in 1999 by Richardson and coworkers [101] to study the ability of chitosan to protect against DNase degradation revealed that incubation of polyplexes prepared at NIP ratio of 3/1 in the presence of DNase I (8 U, 1 h incubation) protected pDNA from degradation. Other studies of chitosans as gene delivery vehicles confirm that the NIP ratio has to be at least 3/1 to 5/1 in order to provide a sufficient protective effect against DNases. [Pg.151]

Braun CS, Vetro JA, Tomalia DA et al (2005) Structure/function relationships of polyami-doamine/DNA dendrimers as gene delivery vehicles. J Pharm Sci 94(2)423 136... [Pg.182]

Pun SH, Davis ME (2002) Development of a nonviral gene delivery vehicle for systemic application. Bioconjug Chem 13 630-639... [Pg.189]

Clamme JP, Krishnamoorthy G, Mely Y (2003) Intracellular dynamics of the gene delivery vehicle polyethylenimine during transfection investigation by two-photon fluorescence correlation spectroscopy. Biochim Biophys Acta 1617 52-61... [Pg.303]

Regarding safety concerns, non-viral gene delivery vehicles that have the required efficiency and safety for use in human gene therapy are being widely... [Pg.223]

The last talk in this session, by Andrew Lyddiatt (University of Birmingham, United Kingdom), showed how liquid-liquid extraction can address some of the problems associated with the purification of nanoparticulates (e.g., viral and nonviral gene delivery vehicles). Nanoparticles (particle size range 20-150 nm) with low diffusivity and low molalities in culture feedstocks pose unique process engineering problems in the design and implementation of selective recovery and formulation operations. This paper demonstrated how aqueous two-phase partition systems (polymer-polymer and polymer-salt) can circumvent the process bottlenecks posed by the use of... [Pg.704]

Leong KW, Mao HQ, Truong-Le VL, Roy K, Walsh SM, August JT (1998) DNA-polycation nanospheres as non-viral gene delivery vehicles. J Control Release 53 183-193... [Pg.58]

OTHER COLLOIDAL SYSTEMS Gene Delivery Vehicles... [Pg.1065]

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