Plasmid broad host range

C. Winstanley. J. A. W. Morgan, R. W. Pickup, J. G. Jones, and J. R. Saunders, Differential regulation of Lambda pi, and Pk promoters by a cl repressor in a broad-host-range thermoregulated plasmid marker system. Appl. Environ. Microbiol., 55 771 (1989). 

Antoine, R. and Locht, C. (1992) Isolation and molecular characterization of a novel broad-host-range plasmid from Bordetella bronchiseptica with sequence similarities to plasmids from Gram-positive organisms. Molecular Microbiology, 6, 1785-1799. 

Tao, L., Jackson, R.E. and Cheng, Q. (2005) Directed evolution of copy number of a broad host range plasmid for metabolic engineering. Evolutionary Engineering, 7, 10-17. 

Mob, can be mobilized during conjugation with broad-host-range plasmids Xm, kanamycin resistance Km, kanamycin sensitivity Cm, chloramphenicol Sm, streptomycin 7c, tetracycline. 

Powell B, Mergeay M, Christofi N. 1989. Transfer of broad host-range plasmids to sulphate-reducing bacteria. FEMS Microbiol Lett 59 269-74. 

Isolation and sequencing of the cellulose synthase gene(s) has not been accomplished yet however, DNA from Acetobacter xylinum containing this gene(s) was cloned into broad host-range plasmid vectors (82). These vectors were mobilized into Pel- mutants to test for complementation. To date, this approach has not produced a pellicle-forming transconjugant from a Pel- mutant of Acetobacter (82). The direct correlation between cellulose production and presence of plasmid DNA in Acetobacter has been reported... 

The aromatic degradative pathways receiving the most attention include those for toluene, benzoate, PCBs, and naphthalene. Five completely independent routes of toluene catabolism have been well characterized for aerobic bacteria (Figure 11.1). To date, all five pathways have been described in Pseudomonas and Burkholderia, despite the fact that at least two have been shown to be encoded by broad-host-range degradative plasmids. 

GEM slowly declined in number from start until total disappearance by day 70. Transconjugants of indigenous population persisted and spread. Measured conjugation frequencies of broad host range plasmids to indigenous microflora. 

Naik, G. A., Bhat, L. N., Chopade, B. A. Lynch, J. M. (1994). Transfer of broad-host-range antibiotic resistance plasmids in soil microcosms. Current Microbiology, 28, 209-15. 

Resistance can emerge by mutation, though more commonly it is due to plasmid-encoded trimethoprim-resistant dihydrofolate reductases. These resistant enzymes may be located within transposons on conjugative plasmids that exhibit a broad host range, accounting for rapid and widespread dissemination of trimethoprim resistance among numerous bacterial species. 

T Kieser, DA Hopwood, HM Wright, CJ Thompson. pIJlOl, a multi-copy broad host-range Streptomyces plasmid functional analysis and development of DNA cloning vecters. Mol Gen Genet 185 223-238, 1982. 

Inducible resistance to high levels of vancomycin in enterococci is mediated by transposon Tn 1546 or related transposons [262, 263]. The transposition of Tn 1546 into plasmids with a broad host range or into con-jugative transposons would enable resistance to spread to 5. aureus which can exchange genetic information with enterococci [260]. Plasmid-mediated resistance to vancomycin has been successfully conjugated, in laboratory experiments, from enterococci to S. aureus, provided that erythromycin (6) and not vancomycin was used as the selective agent [265], It was only a question of time before such an event was shown to occur clinically [266, 267] and vancomycin resistance has now been found in an MRSA strain [268]. The seriousness of this cannot be over-emphasized. 

Construct a large-insert genomic library in a broad host range plasmid. This will facilitate the cloning of CO-shift related genes as well as be useful in complementation experiments when a CO-shift mutant is obtained. 

Gram-positive bacteria are made bioluminescent (e.g., S. aureus Xen-29) through the stable integration of an optimized P. luminescense luxABCDE cassette onto their chromosome as first described by Francis et al. (3). This broad host range plasmid can be used to transform a variety of Grampositive bacteria to a stable bioluminescent phenotype. However, the procedure described below is optimized for the transformation of S. aureus. 

The pet (fbc) operons of various Inh mutants were cloned into the broad host-range plasmid pRK404 by digestion of the chromosomal DNA of their Kan derivatives with appropriate restriction enzymes that cut outside... 

Plasmids of the incompatibility groups C, N, P, Q and W exhibit a host range much widm than those commonly used as iq>licons for E. co/l-specific vectors. Their properties have been reviewed in several recent articles that provide lists of vectors and hosts (34,48,49). Vectors based on IncP and IncQ replicons are among the most highly developed broad-host-range vectors available to date. For some of them, such as pMMB66 and pMMB207 smes the entire nucleotide sequence has been determined (34). 

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