Plants cloning

LANGE, B.M., CROTEAU, R., Isoprenoid biosynthesis via a mevalonate-independent pathway in plants cloning and heterologous expression of 1-deoxy-D-xylulose 5-phosphate reductoisomerase from peppermint, Arch. Biochem. Biophys., 1999,365,170-174. 

Fig. 7.11 Schematic diagram of foreign gene expression in plant. P promoter, T terminator, pGA482 plant cloning vector, Km kanamycin resistance gene, Tc tetracycline resistance gene.

Plant Clone name Observations Inducibility by other factors References... 

Another major application of herbicide resistance is its utility as a selectable marker. Like antibiotic resistance in bacterial transformation, herbicide resistance should prove extremely useful for selecting transformants that are insect resistant, disease resistant, or engineered for other non-selectable traits. Many of these herbicide resistant markers have already been integrated into plant cloning vectors (32.43). 

Natural cloning is more common among plants. Clones of single parent plants can arise from bulbs, spores, stems, and leaves. Plant breeders may develop a superior variety of apple (Red Delicious) and then, by producing millions of genetically identical trees that grow identically delicious apples, establish a successful fruit market. 

GENSCHEL, U., POWELL, C.A., ABELL, C., SMITH, A.G., The final step of pantothenate biosynthesis in higher plants Cloning and characterization of pantothenate synthetase from Lotus japonicus and Oryza sativum (rice), Biochem. J., 1999,341,669-678. 

The Important Sequence Model module does sensitivity studies and importance rankings for about a thousand highest frequency sequences. The analyst zooms to the most frequent plant damage category, to the most frequent sequences in that category, to the most important top event, to the most important split fraction, and to the most important cutsets. If sensitivity analysis is needed on the model as a whole, a menu option, "CLONE a Model," makes a copy of the model, c hange,s are made, and results compared. 

At present, cell culture work is done mostly by hand by horticulturists in large greenhouses (Plate 3). Chemical engineers could greatly increase the usefulness of this method of plant propagation by developing efficient automated processes for producing plants from cloned cells. 

Rebmann G. Hertig C. Bull J. Mauch F. Dudler R. (1991) Complementary DNA cloning and sequence analysis of a pathogen-induced putative peroxidase from rice / / Plant Mol. Biol. V. 16. P. 329-331. 

Roberts E. Kutchan T. Kolattukudy P. E. (1988) Cloning and sequencing of cDNA for a highly anionic peroxidase from potato and the induction of its mRNA in suberizing potato tubers and tomato fruits / / Plant Mol. Biol. V. 11. P. 15-26... 

CAM is unlikely to be useful for any of the conventional food crops. Nevertheless, further study of those plants in which CAM is inducible could prove useful since isolation of, for example, the PEP carboxylase gene will permit the isolation of its controlling sequences. This will provide us with another set of stress-specific (drought) promoters and enhancers. Furthermore the PEP carboxylase gene has proved amenable to cloning via the cDNA route (Harpster Taylor, 1986). 

Czarnecka, E., Edelman, L., Schoffl, F. Key, J.L. (1984). Comparative analysis of physical stress responses in soybean seedlings using cloned heat shock cDNAs. Plant Molecular Biology, 3, 45-58. 

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