Plant transformation vectors

Bevan M (1984) Binary Agrobacterium vectors for plant transformation. Nucl Acid Res 12 8711-8721... 

McBride, K. Summerfelt, K. (1990). Improved binary vectors for Agrobacterium-mediated plant transformation. Plant Molecular Biology 14, 269-76. 

Yemets, A., Radchuk, V., Bayer, O., Bayer, G., Pakhomov, A., Baird, W. V., and Blume, Y. B. (2008). Development of transformation vectors based upon a modified plant a-tubulin gene as the selectable marker. Cell Biol. Int. 32,566-570. 

Another major application of herbicide resistance is its utility as a selectable marker. Like antibiotic resistance in bacterial transformation, herbicide resistance should prove extremely useful for selecting transformants that are insect resistant, disease resistant, or engineered for other non-selectable traits. Many of these herbicide resistant markers have already been integrated into plant cloning vectors (32.43). 

The E. coli mutant EPSPS gene was engineered into a plant expression vector (pMON8078) and used for transformation of tobacco leaf discs. In this construct, the mutant EPSPS gene was driven by the CaMV 35S promoter and the 3 -polyadenylation signal was derived from the nopaline synthase gene. 

Plant transformation techniques allow the delivery of the transforming DNA through the cell wall and plasma and nuclear membranes, without compromising the viability of the cell. Gene delivery can be performed either via a biological vector (plant viruses or bacteria) or by non-biological vector-free procedures (chemical methods, microinjection, particle bombardment, etc.) (Birch 1997). 

There is a bewildering variety of methods used for obtaining plants transformed by Agrobacterium vectors. This variation is due to the greatly different responses of plants to antibiotic... 

Among 34 sense transgenic lines of tobacco generated, 16 overexpressed PLD activity at least 20-fold higher than control plants (transformed with vector alone). The overexpressed castor PLD in tobacco was readily detectable by measurements of PLD... 

Plant transformation Wild type (WT) A. thaliana plants of ecotype Columbia were transformed in planta by wound inoculation (5) with a suspension of A. tumefaciens strain GV3101 bearing helper nopaline plasmid pMP90 and binary vector p5XC7-7 /pRD400. B. napus cv. Hero (high erucic acid variety) was transformed by co-cultivation of hypocotyl explants using modifications of a published protocol (6). 

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