Planar chromatography horizontal developing chamber

FIGURE 6.10 The cover of the prepared chromatoplate for gradient development in a fully online horizontal chamber 1 — solvent system inlet, 2 — Silcoflon cover sheet, 3 — chromatoplate, 4 — solvent system outlet, 5 — channel for solvent system. (From Nyiredy, Sz. and Benko, A., Proceedings of the International Symposium on Planar Separations, Planar Chromatography 2004, Nyiredy, Sz., Ed., Research Institute for Medicinal Plants, Budakalasz, 2004, pp. 55-60. With permission.)... 

Isocratic linear development is the most popular mode of chromatogram development in analytical and preparative planar chromatography. It can be easily performed in horizontal chambers of all types. The mobile phase in the reservoir is brought into contact with the adsorbent layer, and then the movement of the eluent front takes place. Chromatogram development is stopped when the mobile phase front reaches the desired position. Usually 20 X 20 cm and 10 X 20 cm plates are applied for preparative separations, and this makes the migration distance equal to about 18 cm. Due to the fact that the migration distance varies with time according to the equation Z, = (Z, c, and t are the distance of the solvent front traveled, constant,... 

Conventional thin-layer chromatography (TLC) in our experience, known under the name planar chromatography, uses horizontal or vertical glass or Teflon chambers for the development of chromatograms. As stationary phases, commonly known adsorbents or supports based on silica gel, aluminium oxide, magnesium silica, cellulose, and so forth are used particle sizes are about 20 jitm. The migration of the mobile phase is based on the phenomenon of capillary forces. This chromatographic method is described, in detail, in other sections of this volume. 

Vajda, J., Leisztner, L., Pick, J., and Anh-Tuna, N. (1986). Methodological developments in overpressured layer chromatography. Chromatographia 21 1.32-156. Waksmundzka-Hajnos, M., and Wawrzynowicz, T. (1994). Optimization of the separation of plant extracts with the aid of the horizontal DS chamber. J. Planar Chromatogr.—Mod. TLC 7 58-62. 

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