Phthalate dioxygenase

J Li, MR Nelson, CY Peng, D Bashford, L Noodleman. Incorporating protein environments in density functional theory A self-consistent reaction field calculation of redox potentials of [2Ee2S] clusters in feiTedoxm and phthalate dioxygenase reductase. J Phys Chem A 102 6311-6324, 1998. 

RR spectra of the Rieske protein from T. thermophilus (TRP) and of phthalate dioxygenase from Burkholderia cepacia (PDO) have been reported by Kuila et al. (66, 67), and those of the Rieske protein from Sulfolobus sp. strain 7 tentatively called sulredoxin by Iwasaki et al. (68). Although no complete analysis is yet available, several conclusions can be drawn from these spectra, in comparison to the spectra of proteins containing a 4-cysteine coordinated [2Fe-2S] cluster (Table VI). 

X-ray absorption spectroscopy has been performed on the isolated Rieske protein from bovine heart mitochondrial bc complex 69) as well as on the Rieske-type cluster in Burkholderia cepacia phthalate dioxygenase (PDO) (72). The analysis performed by Powers et al. 69) was significantly hampered by the fact that the presence of two histidine ligands was not fully recognized therefore, only the results obtained with the dioxygenase where the mononuclear iron has been depleted will be considered here. Table VII gives a comparison of the distances obtained from the fit of the EXAFS spectra assuming an idealized Rieske model and of the distances in the crystal structures... 

ENDOR and ESEEM studies of phthalate dioxygenase (PDO) (7, 84), benzene dioxygenase (85), 2-halobenzoate 1,2-dioxygenase (86), 2,4,5-trichlorophenoxyacetate monooxygenase (86a), spinach bef complex (8), and the bci complexes from Rhodobacter capsulatus (7, 84) and in bovine mitochondrial membranes (87) (Fig. 16) have identified two nitrogen nuclei coupled to the [2Fe-2S] cluster with isotropic N... 

PDO, phthalate dioxygenase BDO, benzene dioxygenase 2,4,5-T-MO, 2,4,5-trichlorophenoxyacetate monooxygenase UHDBT, undecyUiydroxybenzothiazole. 

Phthalate dioxygenase (PDO) Conflicting values have been quoted [—60 mV at pH 6.9 in an absorption monitored titration performed at 10°C Kuila and Fee (129) —120 mV at pH 7.0 Correll etal. (229)] but experimental details have not been reported. 

An iron sulfur-flavoprotein that transfers electrons directly to the dioxygenase, as in phthalate dioxygenase (class I)... 

The structure of phthalate dioxygenase reductase that transfers electrons directly from NADPH to phthalate dioxygenase has been determined by X-ray crystallography (119). In class II or class III dioxygenases, the ferredoxin obligately transfers electrons from the reductase to the terminal dioxygenase (64a) it can be either a Rieske-type ferredoxin or a ferredoxin containing a 4-cysteine coordinated [2Fe-2S] cluster. 

I, 2-diol and NAD. This multiprotein complex contains an iron-sulfur flavoprotein, an iron-sulfur oxygenase, and ferredoxin. See also Phthalate Dioxygenase... 

BENZENE 1,2-DIOXYGENASE PHTHALATE DIOXYGENASE BENZOATE-CoA LIGASE BENZOATE 1,2-DI OXYGENASE BENZOYLFORMATE DECARBOXYLASE BERBERINE BRIDGE ENZYME BETA (j8)... 

Phthalate dioxygenase, 47 119 ENDOR and ESEEM studies, 47 132 spin-spin interactions, 47 466 Phthalate dioxygenase reductase, structure, 47 150... 

Abbreviations BCA II bovine carbonic anhydrase II SOD superoxide dismutase TRN transferrin AP alkaline phosphatase Pj inorganic phosphate PDO phthalate dioxygenase E = empty . 

FIGURE 11. stereo diagram of phthalate dioxygenase reductase. The FMN- and NADPH-binding domains are on the top and the 2Fe-2S binding domain is on the bottom. The FMN and 2Fe-2S prosthetic groups are shown as skeletal models. 

Correll, C. C., Batie, C. J., Ballou, D. P., and Ludwig, M. L., 1992, Phthalate dioxygenase reductase a modular structure for electron transfer from pyridine nucleotides to [2Ee-2S], Science 258 1604nl610. 

Gurbiel, R. J., Batie, C. J., Sivaraja, M., True, A. E., Fee, J. A., Hoffman, B. M., and Ballou, D. P., 1989, Electron-nuclear double resonance spectroscopy of 15N-enriched phthalate dioxygenase from Pseudomonas cepacia proves that two histidines are coordinated to the [2Fe-2S] Rieske-type clusters, Biochemistry 28 4861n4871. 

JF Cline, BM Hoffmann, WB Mims, E La Haie, DP Ballou and JA Fee (1985) Evidence forN coordination to Fe in the [2Fe-2S] clusters of Thermus Rieske protein and phthalate dioxygenase from Pseudomonas. J Biol Chem 260 3251-3254... 

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