Phosphate group cyclic ester formation

The alcoholysis of the cyclic phosphate of catechol by alditols can lead, after acid hydrolysis of intermediate, cyclic phosphates, to the selective formation of phosphoric esters of the primary hydroxyl groups in the alditols. Thus, erythritol and D-mannitol afford, after chromatographic purification of the reaction products, their 1-phosphates in yields of 31 and 38%, respectively.217 The method was used to convert riboflavine into riboflavine 5 -phosphate.218 1-Deoxy-1-fluoro-L-glycerol has been converted into the 3-(dibenzyl phosphate) in 54% yield by selective reaction with dibenzyl phosphorochloridate. 219... 

Ribonuclease T2 is regarded as a nonspecific endoribonuclease [EC 2.7.7.17, ribonucleate nucleotido-2 -transferase (cyclizing)]. It preferentially splits the internucleotide bonds between the 3 -adenylic acid group and the 5 -hydroxyl group of adjacent nucleotides in RNA, with the intermediary formation of adenosine 2, 3 -cyclic phosphate and splits consequently all secondary phosphate ester bonds of other nucleotides in RNA via the nucleotides 2, 3 -cyclic phopshates. 

The formation of vanadate esters with hydroxyl groups in aqueous solutions has been studied in detail for methanol (53), ethylene glycol (57), phenol, and tyrosine (58). Vanadate is able to form cyclic complexes when there are adjacent hydroxyls in the molecule (59) and interacts with luidine, adenosine monophosphate (60), glutathione disulphide (61), and phosphate (62). Rehder (63) studied the interaction of amino acids and dipeptides with vanadate. He concluded that complexes are formed in which the peptide function and the iV-terminal amino group are involved. Similarly, it was reported (64) that vanadate... 

---