2-Phenyl-6-sulfo

Amin (4-Nitro-phenyl)-phenyl-sulfo- (Na-Salz) XI/2, 672... 

CjgHi gS I>imethyl-r2-athoxy-phenyl]-sulfo niumhydroxw 6 1 396. 

Phenyl sulfo nitroxide CgHsNO + HSO J + PbOj/ 74Banl... 

Zincon (o-[l-(2-bydroxy-5-sulfo)-3-phenyl-5-formazono]-benzoic acid) [135-52-4] M 459.4. Main impurities are inorganic salts which can be removed by treatment with dilute acetic acid. Organic contaminants are removed by refluxing with ether. It can be recrystd from dilute H2SO4. [Fichter and Schiess Chem Ber 33 751 1900.]... 

Turner, S.R., Walker, T.O. and Thaler, W.A. "Sodium Styrene Sulfonate Co-Sodium N-(4-Sulfo Phenyl) Maleimide An Improved Viscosity Control Additive," US Patent 4,478,727(1984). 

Figure 5.20 Sulfo-HSAB is a short photoreactive crosslinker that can be used to modify amine-containing molecules through its NHS ester end to form amide linkages. After photoactivation, the phenyl azide group can react with amines to create a covalent bond.

Figure 5.25 The reaction of sulfo-SAPB with an amine group is done first to form an amide bond derivative through its NHS ester end. Subsequent exposure to UV light causes the phenyl azide group to ring-expand to a highly reactive dehydroazepine, which can couple to nucleophiles, such as amines.

Sulfo-SAMCA, sulfosuccinimidyl-7-azido-4-methylcoumarin-3-acetate, is a heterobifunctional reagent similar in design to SAED (Section 3.9, this chapter) (Thermo Fisher). One end of the crosslinker contains an amine-reactive sulfo-NHS ester, while the other end is an AMCA derivative (Chapter 9, Section 3) that contains a photosensitive phenyl azide group. Unlike... 

Since the active ester end of the molecule is subject to hydrolysis (half-life of about 20 minutes in phosphate buffer at room temperature conditions), it should be coupled to an amine-containing protein or other molecule before the photolysis reaction is done. During the initial coupling procedure, the solutions should be protected from light to avoid decomposition of the phenyl azide group. The degree of derivatization should be limited to no more than a 5- to 20-fold molar excess of sulfo-SBED over the quantity of protein present to prevent possible precipitation of the modified molecules. For a particular protein, studies may have to be done to determine the optimal level of modification. 

Figure 28.8 The heterobifunctional crosslinkers sulfo-SAND, SANPAH, and sulfo-SANPAH contain an amine-reactive (sulfo)NHS ester on one end and a photoreactive phenyl azide group on the other end. Sulfo-SAND allows release of conjugates by reduction of its internal disulfide bridge.

Sulfo-SAND, SANPAH, and Sulfo-SANPAH all contain a nitrated phenyl azide photoreactive group. The presence of the nitro group shifts the optimal wavelength for photoactivation... 

Figure 28.11 Sulfo-SBED is a label transfer agent that contains a water-soluble sulfo-NHS ester to label bait proteins and a phenyl azide group for photoreactive capture of a prey protein. The biotin label can be used for detection or isolation of protein-protein conjugates using (strept)avidin reagents. The stars indicate the atoms that were used to measure the indicated molecular dimensions.

Figure 28.12 Sulfo-SBED first is used to label a bait protein through reaction of the sulfo-NHS ester with available amine groups on the protein, yielding an amide bond linkage. This labeled bait protein then is added to a sample containing proteins that potentially could interact with the bait. After an incubation period, the sample is exposed to UV light to photoactivate the phenyl azide group. This reaction causes any interacting prey proteins to be crosslinked with the bait protein, forming a complex containing a biotin affinity tag.

Phenyltrimethylammonium sulfo-methylate, 53, 111 Phenyltrimethylammonium tribromide, selective bromina-tion with, 53, 112, 114 4-Phenylurazole, from 4-phenyl-1-carbethoxysemicarbazide and potassium hydroxide,... 

Amino acid Naphthalene-2-sulfo- Phenyl silica 50 mM Phosphoric acid 14... 

Carboxy-5-kydroxy- -(4-sulfo-phenyl)-4- (4-sulfo-phenylazo )-pyrrazol-Trinatrium-Salz... 

PY N = N-C6H5 NaOCI /N—CbHb HO3S 2-Phenyl-6-sulfo-... E8 ... 

Binaphthalenel-4,4, 7,7 -tetrasulfonic acid, 2,2 -bis[[bis(3-sulfo-phenyl)phosphino]methyl]-, octasodium salt, 32 16-17... 

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