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Sulfo-SAMCA

Sulfo-SAMCA, sulfosuccinimidyl-7-azido-4-methylcoumarin-3-acetate, is a heterobifunctional reagent similar in design to SAED (Section 3.9, this chapter) (Thermo Fisher). One end of the crosslinker contains an amine-reactive sulfo-NHS ester, while the other end is an AMCA derivative (Chapter 9, Section 3) that contains a photosensitive phenyl azide group. Unlike [Pg.319]

Cleavage of crosslink with transfer of fluorescent group to second protein [Pg.320]

Sulfo-SAMCA Sulfosuccinimidyl-7-azido-4-methylcoumarin-3-acetate MW 458.34 [Pg.322]

Sulfosuccinimidyl 7-azido-4-methylcoumain-3-acetate (sulfo-SAMCA) is a heterobifunctional reagent similar in design to SAED (this chapter. Section 3.9) (Pierce). One [Pg.270]

Cleavage of Cross-link with Transfer of Fluorescent Group to Second Protein [Pg.271]

See other pages where Sulfo-SAMCA is mentioned: [Pg.319]    [Pg.321]    [Pg.321]    [Pg.322]    [Pg.290]    [Pg.292]    [Pg.293]    [Pg.270]    [Pg.272]    [Pg.273]   


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4 -sulfo

Amines sulfo-SAMCA

SAMCA

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