Phaseolus vulgaris purification

Rodgers, M.W. and Bolwell, G.P. (1992) Partial purification of golgi-bound arabinosyltransferase and two isofotms of xylosyltransferase from french bean Phaseolus vulgaris L.). BiochemJ. 288 817-822. 

Polymeric procyanidin extraction from black beans (Phaseolus vulgaris L. cv. Black Turtle Soup) and purification was a... 

J. J. Marshall and C. M. Lauda, Purification and properties of phaseolamin, an inhibitor of a-amylase, from the kidney bean, Phaseolus vulgaris, J. Biol. Chem., 250 (1975) 8030-8037. 

The lectin from Phaseolus vulgaris L. (var. red), isolated by conventional protein-purification procedures, was shown to be localized in the cytoplasm of the cotyledon and embryo of the seed.243e A glycoprotein (6.6% carbohydrate), the lectin had molecular weight 128,000, and contained high proportions of aspartic acid, serine, and tryptophan, but no cysteine.243e... 

Smith C, Bolwell GP (1997) Purification of an elicitor-induced glucan synthase (callose synthase) from suspension cultures of French bean [Phaseolus vulgaris L.) purification and... 

Purification of carcinoembryonic antigen Purification of D-glucocerebrosidase Purification of Phaseolus vulgaris phyto-haemagglutinin... 

Affinity chromatography of human liver hydrolases Fractionation of low molecular weight porcine heparin into active and relatively inactive forms Affinity chromatography of heparin Affinity chromatography of rabbit anti-thyroglobulin antibodies Purification of Phaseolus vulgaris isolectins Purification of in vivo activity of an H-Z allo-antiserum... 

Multiple forms of cysteine synthase have been demonstrated in a number of plants. Two peaks of cysteine synthase were obtained by ammonium sulfate fractionation of acetone powders of spinach (Giovanelli and Mudd, 1968). A highly purified cysteine synthase from rape leaves was resolved into two fractions by polyacrylamide gel electrophoresis. The two had similar sizes and subunit compositions but one contained pyridoxal phosphate, whereas the other contained a derivative of pyridoxal phosphate (Masada et al., 1975). The electrophoretic mobility of the latter enzyme could be converted to that of the former by incubation with pyridoxal phosphate. Two forms of cysteine synthase have been extracted from both Phaseolus vulgaris and P. polyanthus (Table II) (BertagnoIIi and Wedding, 1977). It is unlikely that the multiple forms of the Phaseolus enzyme are due to modification of the pyridoxal phosphate prosthetic group, since pyridoxal phosphate was included in all media used for purification and assay of these forms. 

Two forms of a-D-mannosidase from yellow wax beans Phaseolus vulgaris) have been purified by immunoadsorption. A high degree of immunological identity has been demonstrated between a-D-mannosidase I and II from P. vulgaris. This made possible the purification of both forms of the enzyme by chromatography on an immunoadsorbent prepared from anti-fa-D-mannosidase I) antiserum. 

Purification of two forms of a-D-manno-sidase from Phaseolus vulgaris by immunoadsorption... 

MacCormack B.A., Gregory A.C., Kerry M.E., Smith C., and Bolwell G.P. 1997. Purification of an elicitor-induced glucan synthase (callose synthase) from suspension cultures of French bean (Phaseolus vulgaris L.) purification and immunolocation of a probable M(r)-65,000 subunit of the enzyme. Planta 203 196-203. 

Stroma, erythrocyte membrane residues from haemolysed red cells, have been intra-crosslinked with glutaraldehyde and used for the affinity chromatographic purification of two nonspecific and noninhibitable lectins fi-om Phaseolus vulgaris and P. coccineus. Formaldehyde-fixed human erythrocytes have provided a specific adsorbent for the purification of the lectin from Robinia pseudocacia. ... 

Purification of specific anti-(2,4-dinitrophenyl) antibodies by immunoadsorption Purification of Datura stramonium phytohaemagglutinin by affinity chromatography Affinity chromatography of Phaseolus vulgaris phytohaemaglutinin... 

Kidney beans (Phaseolus vulgaris) contain a proteinaceous inhibitor of a-amylase named phaseolamin . After purification by heat treatment, ion-... 

Matsuda H, Tanaka G, Morita K, Hirayama O. Purification of a lipolytic acyl-hydrolase from Phaseolus vulgaris leaves by affinity chromatography on palmitoylated gause and its properties. Agric Biol Chem 1979 43 563-570. 

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