Phaseolus vulgaris isolation

Pipecolic acid has been isolated and identified by various researchers from several legumes, hops, mushrooms, potato tuber, green pepper, tulip, celery, asparagus, Rhodesian teak, barley, and coconut milk. Grobbelaar et al. 58) isolated large quantities of free (-)pipe-colic acid from Phaseolus vulgaris. 

Bialek K, Cohen JD. Isolation and partial characterization of the major amide-linked conjugate of indole-3-acetic acid from Phaseolus vulgaris. Plant Physiol 1986 80 99-104. 

Howell, R. K. Influence of air pollution on quantities of caffek acid isolated from leaves of Phaseolus vulgaris. Phytopathology 60 1626-1629, 1970. 

Lightfoot, D.A., Green, N.K. Cullimore, J.V. (1988). The chloroplast-located glutamine synthetase of Phaseolus vulgaris L. Nucleotide sequence, expression in different organs and uptake into isolated chloroplasts. Plant Molecular Biology 11, 191-202. 

West, C.A., Phinney, B.O. "Gibberellins from flowering plants. I. Isolation and properties of a gibberellin from Phaseolus vulgaris L." J.A.C.S., 1959, 81, 2k2h-2k27. 

O-Xylopyranosyl zeatin (46), a new O-glycosylcytokinin, has been isolated from Phaseolus vulgaris <87PNA(84)3714> and synthesized (8% yield) <87JCR(S)iio> by reaction of the O-xylosylamine (45) with 6-chloropurine (Equation (4)). 

An alternative approach involves insolubilized glycoproteins. Thus, an affinity system for the isolation of the lectin of red kidney-bean (Phaseolus vulgaris)149,154 involved thyroglobulin-Sepharose, and, for... 

The lectin from Phaseolus vulgaris L. (var. red), isolated by conventional protein-purification procedures, was shown to be localized in the cytoplasm of the cotyledon and embryo of the seed.243e A glycoprotein (6.6% carbohydrate), the lectin had molecular weight 128,000, and contained high proportions of aspartic acid, serine, and tryptophan, but no cysteine.243e... 

The intermediacy of prephytoene pyrophosphate (89) was first shown by Altman and coworkers who identified this compound as a product of the incubation of geranylgeranyl pyrophosphate (66) with an extract of photoinduced Mycobacterium sp. The direct incorporation of geranylgeranyl pyrophosphate (66) into phytoene (88) has been observed in various systems including a soluble tomato plastid enzyme systemchloroplasts isolated from Phaseolus vulgaris cell-free extracts of P. blakesleeanus and... 

In 1987 Kim et al. isolated 25-methyldolichosterone (37, Figure 2.26) from immature seeds of Phaseolus vulgaris, and found it to be about one order of magnitude more bioactive plant-growth regulator than... 

As a result, 25-methylbrassinolide (40) was found to be more bioactive than the natural brass-inolide. Later, 25-methyl-2,3-diepidolichosterone (38) was isolated from Phaseolus vulgaris. 25-Methyldolichosterone (37) and 25-methylcastasterone (39) were also synthesized.81... 

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