Pharmaceutical preparation fluorescence detection

Perez-Ruiz, T., Martinez-Lozano, C., and Galera, R. (2006). Development and validation of a capillary electrophoresis method with laser-induced fluorescence detection for the determination of captopril in human urine and pharmaceutical preparations. Electrophoresis 27(12), 2310—2316. 

Zgorka, G. Dawka, S. 2001. Application of conventional LW, photo-diode array (PDA) and fluorescence (FL) detection to analysis of phenolic acids in plant material and pharmaceutical preparations. J. Pham. Biomed. Anal. 24 1065-1072. 

It was shown that the chromatography method was suitable to separate recombinant EPO from amounts of human serum albumin commonly present as a stabilizer in various pharmaceutical preparations. In addition, it was possible to obtain different elution profiles for EPO products with variations in the glycoforms. Fluorescence detection was applied for quantification and showed linear signals over the range of 10-200-pg/mL EPO. 

He, D., Chena, B., Tiana, Q., Yaoa, S. (2009). Simultaneous determination of five anthraquinones in medicinal plants and pharmaceutical preparations by HPLC with fluorescence detection. /, Pharm. Biomed. Anal, 49, pp. 1123-1127, ISSN 0731-... 

Hoebus et al. (1993) used silica gel TLC with various mobile phases to identify corticosteroid hormones available on the European market. Detection was by fluorescence after the plates were sprayed with alcoholic sulfuric acid and heated. Datta and Das (1994) identified and quantified corticosteroids and their esters in pharmaceutical preparations of creams and ointments. The preparations were dissolved in chloroform, centrifuged to remove water and insoluble material, and silica gel TLC with hexane mobile phase was used to wash out base ingredients followed by chloroform-ethyl acetate (1 1) for free steroids or (2 1) for esters and spectrodensitometry at 240 nm recoveries were 99 1% and the agreement of results with the official methods used was excellent. 

Pharmaceutical preparations containing riboflavin have been analyzed by TLC using concentrated ethanolic extracts on silica gel plates developed in butanol-benzene-acetic acid-water (8 7 S 3) or butanol-acetic acid-water (9 4 5) (7). Foods, tissue samples and urine each require particular methods of sample preparation and these, together with a number of solvent systems have been reviewed (8). A darkroom is required for sample preparation and chromatography of flavins to prevent photolytic degradation. The fluorescent property of flavins provides a convenient means of detection and spots have been located under radiation at 254 and 366nm (4). HPTLC followed by fiberoptic fluorimetry has been used to quantitate riboflavin in vitamin mixtures and can detect 48-320 ng (6). Recently, a method has been described using mixed-layer plates of GDX-102 and silica gel G (1 1) precoated with hexadecyl-trimethylammonium bromide developed in 60-70% ethanol (9). 

The detection limit depends on the method used. Huorometric detection is much more sensitive than its spectrophotometric counterpart. The latter method is suitable for analysis of large quantities of thiamine in pharmaceutical preparations and foods the detection limit is approximately 2 ng or 6 pmol as thiamine hydrochloride. On the other hand, the detection limit by the fluorometric method is <17 pg or 0.05 pmol as thiamine hydrocholoride. The lowest detection limit so far reported for thiamine is 5 fmol, using fluorescence (21,22). Huorescence detection is therefore more suitable for the analysis of thiamine in biological materials such as cells, blood, and urine. 

Takahashi, M. Nagashima, M. Shigeoka, S. Kamimura, H. Kamata, K. Determination of thyroid hormones in pharmaceutical preparations, after derivatization with 9-anthroylnitrile, by high-performance liquid chromatography with fluorescence detection. J. Chromatogr., A 2002, 958, 299-303. 

Analysis of Drugs in Preparations HPLC has found widespread use for the quantitative analysis of drugs in preparations of pharmaceutical and illicit manufacture. Drug concentrations are generally high enough to allow dissolution of the sample (tablet, powder, ointment, etc.) in a suitable solvent followed by injection. Ultraviolet-visible, fluorescence, or refractive index detection is normally used. 

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