Phage vectors, gene cloning

Yanisch-Perron, C., Vieira, J. and Messing, J. 1985. Improved M13 phage cloning vectors and host strains nucleotide sequences of the M13mpl8 and pUCl9 vectors. Gene 33 103-1 19. 

Phage Display Vectors for Cloning of Antibody Genes in Alphabetical Order co... 

A large number of different phage display vectors have been constructed. With pretending to be complete, Table 1 lists a selection of phage display vectors. Some of them have not been used for the construction of a library up to now but have been included because they offer possible alternatives. For example, one of the systems allows the success of antibody gene cloning to be monitored by the expression of green fluorescent protein (52). 

DA Hopwood, MJ Bibb, KF Chater, T Kieser. Plasmid and phage vectors for gene cloning and analysis in Streptomyces. Meth Enzymol 153 116-167, 1987. 

Fig. 1, Organization of cab genes in nuclear DNA from petunia and tomato, showing the tandem and head-to-head arrangement of the genes. Each region of DNA represents a restriction fragment of nuclear DNA cloned in a A phage vector. Redrawn from Dunsmuir et al, [28] and Pichersky et al. [30],...

There are two major kinds of cloning vectors. The first are bacterial virus or phage vectors. These are bacterial viruses that have been genetically altered to allow the addition of cloned DNA fragments. These viruses have all the genes required to replicate 100-200 copies of the virus (and cloned fragment) per infected cell. 

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