Phage vectors

Plasmids are small, circular DNAs that range in size from 2 to approximately 100 kb. They also can be amplified to approximately 20 copies per cell. In addition, plasmids can be introduced into bacterial cells, although less efficiently than phage vectors. Some plasmids can be used to introduce recombinant DNA into yeast cells. 

Development of Filamentous Phage Vectors for Antibody Display... 

Parmley, S and Smith, G (1988) Antibody-selectable filamentous fd phage vectors affinity-purification of target genes Gene 73, 305—318. 

The following protocols have been used for phage vectors, although most of them can be directly applied to phagemids. 

DA Hopwood, MJ Bibb, KF Chater, T Kieser. Plasmid and phage vectors for gene cloning and analysis in Streptomyces. Meth Enzymol 153 116-167, 1987. 

Maruyama IN, Maruyama HI, Brenner S, Afoo A phage vector for the expression of foreign proteins, Proc. Natl. Acad. Sci. USA, 91 8273-8277, 1994. 

Glaser SM, Yelton DE, Huse WD, Antibody engineering by codon-based mutagenesis in a filamentous phage vector system, J. Immunol., 149 3903-3913, 1992. 

Cloning DNA sequences into plasmid and phage vectors... 

Rapid random sequencing by shotgun cloning into single stranded phage vectors (Sanger et al., 1980)... 

Anderson, S., M.J. Gait, L. Mayol and I.G. Young, 1980. A short primer for sequencing DNA cloned in the single-stranded phage vector M13mp2. Nucleic Acids Res. 8, 1731. 

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