Phage protein libraries

Figure 17.10 Construction of a two helix truncated Z domain, (a) Diagram of the three-helix bundle Z domain of protein A (blue) bound to the Fc fragment of IgG (green). The third helix stabilizes the two Fc-binding helices, (b) Three phage-display libraries of the truncated Z-domaln peptide were selected for binding to the Fc. First, four residues at the former helix 3 interface ("exoface") were sorted the consensus sequence from this library was used as the template for an "intrafece" library, in which residues between helices 1 and 2 were randomized. The most active sequence from this library was used as a template for five libraries in which residues on the Fc-binding face ("interface") were randomized. Colored residues were randomized blue residues were conserved as the wild-type amino acid while yellow residues reached a nonwild-type consensus, [(b) Adapted from A.C. Braisted and J.A. Wells,...

Palzkill, T., Huang, W., and Weinstock, G. M. (1998). Mapping protein-ligand interactions using whole genome phage display libraries. Gene 221, 79-83. 

Bach M, et al. Isolation from phage display libraries of lysine-deficient human epidermal growth factor variants for directional conjugation as targeting ligands. Protein Eng 2003 16 1107. 

A number of molecules in groups 2 and 3 have been identified by the differential homing capacity of phage display libraries and combination peptide libraries [71]. Biochemical strategies such as the application of 2D gel electrophoresis on protein extracts from endothelial cell surfaces have also proven useful in this respect [72]. 

Sowa, A., Kordai M.P., Cavanagh, D.R., et al. (2001). Isolation of a monoclonal antibody from a malaria patient-derived phage display library recognising the Block 2 region of Plasmodium falciparum merozoite surface protein-1. Mol. Biochem. Parasitol., 112, 143-147. 

Sheets, M. D., Amersdorfer, P., Finnem, R., Sargent, P., Lindqvist, E., Schier, R., et al. (1998) Efficient construction of a large nonimmune phage antibody library the production of high-affinity human single-chain antibodies to protein antigens. Proc. Natl. Acad. Sci. USA 95, 6157-6162. 

K Johnsson, L Ge. Phage display of combinatorial peptide and protein libraries and their applications in biology and chemistry. Curr Top Microbiol Immunol 243 87-105, 1999. 

Pacios LF, Tordesillas L, Palacin A et al (2011) LocaPep localization of epitopes on protein surfaces using peptides from phage display libraries. J Chem Inf Model 51 1465-1473... 

Adapting techniques based on in vitro protein synthesis to the isolation of enzymes requires establishing a link between a nucleic acid-protein complex and product formation. Methods based on binding, analogous to those developed for phage displayed libraries, may be used to enrich catalysts from noncatalysts. In addition, Tawfik and Griffiths (1998) exploited the aqueous core of reverse micelles as artificial compartments... 

O Neil KT, Hoess RH, Jackson SA, Ramachandran NS, Mousa SA, DeGrado WF, Identification of novel peptide antagonists for GPIIb/Illa from a conformationally constrained phage peptide library, Proteins Structure, Function Genet., 14 509-515, 1992. 

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