Human epidermal growth factor

The antibody of human epidermal growth factor (EGF) was coupled to diol silica (prepared from silica and 3-glycidoxypropyltrimethoxysilane via an epoxy silica) by means of CDI. This supported antibody was utilized for immunoaffinity HPLC analysis of human epidermal growth factor. 213 ... 

Wolff AC, Hammond MEH, Schwartz JN, et al. American Society of Clinical Oncology/College of American Pathologists Guideline Recommendations for Human Epidermal Growth Factor Receptor 2 Testing in Breast Cancer. Arch. Pathol. Lab. Med. 2007 131 18-43. 

Figure 7.3 The ER 1D5 peptide only binds to the ER 1D5 mAb (upper left and lower right). Other mAbs do not bind. The antibody abbreviations in the lower panel are Her2 11G5, and 9C2, human epidermal growth factor receptor type 2 clones 11G5 and 9C2 Mela-HMB45, melanocyte-specific antibody clone HMB45 Vimen V9, vimentin clone V9 Anti-LCA, anti-leukocyte common antigen clones PD7/26 and 2B11, combined as a cocktail Mouse poly IgG, mouse polyclonal IgG. Reproduced with permission from Sompuram et al.7...

Wolff A, Hammond M, Schwartz J, et al. American Society of Clinical Oncology/ College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer. J. Clin. Oncol. 2007 25 118-145. 

Ogiso H, Ishitani R, Nureki O, Fukai S, Yamanaka M, Kim J-H, Saito K, Sakamoto A, Inoue M, Shirouzu M, Yokoyama S (2002) Crystal structure of the complex of human epidermal growth factor and receptor extracellular domains. Cell 110 775-787... 

All the evidence presented in this section concerns aspartic acid residues, and one may wonder whether glutamic acid residues display similar reactivity. The answer is clearly that they do not, in particular for entropy reasons. In fact, replacement of a reactive aspartic acid residue by a glutamic acid residue can greatly increase the chemical stability of a peptide. This is exemplified by human epidermal growth factor (hEGF), an important promoter of... 

C. George-Nascimento, J. Lowenson, M. Borissenko, M. Calderon, A. Medina-Selby, J. Kuo, S. Clarke, A. Randolph, Replacement of a Labile Aspartyl Residue Increases the Stability of Human Epidermal Growth Factor , Biochemistry 1990, 29, 9584-9591. 

Note IL2, interluekin 2 HER2, human epidermal growth factor receptor 2 RSV, respiratory syncytial virus TNF, tumor necrosis factor IgE, Immunoglobulin E EGER, epidermal growth factor receptor VEGF, vascular endothelial growth factor. 

Bach M, et al. Isolation from phage display libraries of lysine-deficient human epidermal growth factor variants for directional conjugation as targeting ligands. Protein Eng 2003 16 1107. 

Trastuzumab is licensed for the treatment of early breast cancer that overexpresses human epidermal growth factor receptor-2 (HER2). It may be administered as monotherapy or in combination with, for example, paclitaxel, docetaxel (taxanes) or anastrozole (aromatase inhibitors). Since trastuzumab can cause cardiotoxicity, concomitant use with anthracyclines such as... 

HER2 human epidermal growth factor receptor-2... 

Herceptin (Trastuzumab, humanized antibody directed against HER2, i.e. human epidermal growth factor receptor 2)... 

Although dehydroalanine formation represents a minor side reaction, the related byproduct may serve to confirm further the assigned disulfide pattern since the Xaa-dehydroalanine bond is stable toward the base treatment. Progress of the oxidative folding of human epidermal growth factor was analyzed by trapping the intermediates via cyanylation followed by their disulfide assignments. 61 ... 

Human epidermal growth factor Triton X-100 (0.02% w/v) Eibronectin (0.05% w/v) Solution 2 fold increased stability at 60°C -1.5 fold stability increase at 60°C [19]... 

Son, K., and C. Kwon, Stabilization of human epidermal growth factor (hEGF) in aqueous formulation. Pharm Res, 1995. 12(3) 451-4. 

GotohN, Tojo A, Hino M et al. A highly conserved tyrosine residue at codon 845 within the kinase domain is not required for the transforming activity of human epidermal growth factor receptor. Biochem... 

Wissner A, Overbeek E, Reich MF et al. Synthesis and structure-activity relationships of 6,7-disubstituted 4-anilinoquinoline-3-carbonitriles. The design of an orally active, irreversible inhibitor of the tyrosine kinase activity of the epidermal growth factor receptor (EGFR) and the human epidermal growth factor receptor-2 (ElER-2). JMed Chem 2003 46 49-63. 

Trastuzumab is a recombinant DNA-derived, humanized monoclonal antibody that binds to the extracellular domain of the human epidermal growth factor receptor HER-2/ . This antibody blocks the natural ligand from binding and down-regulates the receptor. Trastuzumab is approved for the treatment of metastatic breast cancer in patients whose tumors overexpress HER-2/ . As a single agent it induces remission in about 15-20% of patients in combination with chemotherapy, it increases response rate and duration as well as 1-year survival. Trastuzumab is under investigation for other tumors that express HER-2... 

---