Phage libraries cells

Barry MA, Dower WJ, Johnston SA. Toward cell-targeting gene therapy vectors selection of cell-binding peptides from random peptide-presenting phage libraries. Nat Med 1996 2(3) 299-305. 

In conclusion, the ProxiMol technique may enable us to form the maps of multiprotein complexes as it provides both targeting specific cell surface receptors and other proteins associated with this receptor. No matter what technique is used to form phage libraries, it is not possible to interpret the data manually it is accomplished by using bioinformatics. 

The isolation of disease-related antibodies specific for either melanoma [ 180], the autoimmune thyroid peroxidase antigen [174], or neutrophil antigen characteristic of ulcerative colitis [17] from nonnaive patient repertoire phage libraries has been reviewed above in the Panning on cells section. The isolation of anti-viral antibodies with diagnostic or therapeutic potential from antibody repertoire libraries has been described in earlier papers e.g. against HIV [197], hepatitis B virus [198] and human respiratory syncytial virus [199],... 

Cai X, Garen A, Anti-melanoma antibodies from melanoma patients immunized with genetically modified autologous tumor cells, selection of specific antibodies from single-chain Fv fusion phage libraries, Proc. Natl. Acad. Sci. USA, 92 6537-6541, 19. 

FIGURE 19.8 Biopanning procedure on cells. The binding of the phage library, followed by elution and amplification steps, allows the selection of ligands directed against specific cell markers. 

Dissolve the pelleted cells in wash medium and add the phage library (1 x 1013 cfu total phage). Adjust to a final volume of 4 mL. Incubate the cell/phage mixture at 4°C for 3 h on rotation. 

Radosevic, K. Voerman, J. S. Hemmes, A. Muskens, F. Speleman, L. de Weers, M. Rosmalen, J. G. Knegt, P. van Ewijk, W., Colony lift assay using cell-coated filters A fast and efficient method to screen phage libraries for cell-binding clones, J. Immunol. Methods 2003, 272, 219-233... 

The phage library used in most of our selections for cell ligands displays a 20mer peptide at the amino-terminus of the minor coat protein pill (19). We have selected peptides from other phage display libraries (5), including the Ph.D. 12-mer library available from New England Biolabs(MJM and KCB unpublished results). 

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