Peroxidase engineering

Adam, W., Lazarus, M., Saha-Moller, C.R. et al. (1999) Biotransformations with peroxidases, in Biotransformations (ed. K. Faber) Advances in Biochemical Engineering/Biotechnology, vol. 63, Springer-Verlag, pp. 73-108. 

There is huge potential in the combination of biocatalysis and electrochemistry through reaction engineering as the linker. An example is a continuous electrochemical enzyme membrane reactor that showed a total turnover number of 260 000 for the enantioselective peroxidase catalyzed oxidation of a thioether into its sulfone by in situ cathodic generated hydrogen peroxide - much higher than achieved by conventional methods [52],... 

D. Haring and P. Schreier, Chemical engineering of enzymes altered catalytic activity, predictable selectivity and exceptional stability of the semisynthetic peroxidase seleno-subtilisin, Naturwissenschafien 1999, 86, 307-312. 

Flow cytometry is well suited for the analysis of enzyme activity and kinetics at the single cell level (Watson and Dive, 1994). Flow cytometric assays for numerous enzymes including esterases, proteases, peroxidases, lipases, and oxidoreductases3 are available and are widely used in research and clinical practice. To date, flow cytometry has not been widely exploited as a screening tool for enzyme engineering purposes, but this is rapidly changing. 

Miller MA, Geren L, Han GW, et al. Identifying the physiological electron transfer site of cytochrome c peroxidase by structure-based engineering. Biochemistry 1996 35 667-73. 

Huang LS, Wojciechowski G, Ortiz de Montellano PR (2006) Role of heme-protein covalent bonds in mammalian peroxidases - Protection of the heme by a single engineered heme-protein link in horseradish peroxidase. J Biol Chem 281 18983-18988... 

Miyazaki C, Takahashi H (2001) Engineering of the H202-binding pocket region of a recombinant manganese peroxidase to be resistant to H202. FEBS Lett 509 111-114... 

Reading NS, Aust SD (2000) Engineering a disulfide bond in recombinant manganese peroxidase results in increased thermostability. Biotechnol Progr 16 326-333... 

Smith AT, Doyle WA, Dorlet P et al (2009) Spectroscopic evidence for an engineered, catalytically active Trp radical that creates the unique reactivity of lignin peroxidase. Proc Natl Acad Sci USA 106 16084-16089... 

Smith AT, Doyle WA (2006) Engineered peroxidases with veratryl alcohol oxidase activity. Patent (International) WO/2006-114616... 

Bonagura CA, Sundaramoorthy M, Pappa HS et al (1996) An engineered cation site in cytochrome c peroxidase alters the reactivity of the redox active tryptophan. Biochemistry 35 6107-6115... 

Adam W, Lazarus M, Sasha-Moller CR et al (1999) Biotransformations with peroxidases. In Faber K (ed) Advances in biochemical engineering and biotechnology, vol 6. Springer, Berlin... 

Savenkova MI, Newmyer SL, Ortiz de Montellano PR (1996) Rescue of His-42 -> Ala horseradish peroxidase by a Phe-41 -> His mutation. Engineering of a surrogate catalytic histidine. J Biol Chem 271 24598-24603... 

Ozaki S-I, Ortiz de Montellano PR (1995) Molecular engineering of horseradish peroxidase thioether sulfoxidation and styrene epoxidation by Phe-41 leucine and threonine mutants. J Am Chem Soc 117 7056-7064... 

Roncone R, Monzani E, Murtas M et al (2004) Engineering peroxidase activity in myoglobin the haem cavity structure and peroxide activation in the T67R/S92D mutant and its derivative reconstituted with protohaemin-l-histidine. Biochem J 377 717-724... 

Roncone R, Monzani E, Nicolis S, Casella L (2004) Engineering and prosthetic-group modification of myoglobin peroxidase activity, chemical stability and unfolding properties. Eur J Inorg Chem 2203-2213... 

In spite of their catalytic versatility and their capacity to transform a variety of pollutant compounds, peroxidases are not applied at large scale yet. The challenges that should be solved to use peroxidases for environmental purposes have been recently reviewed [146], Three main protein engineering challenges have been identified (a) the enhancement of operational stability, specifically hydrogen peroxide stability (see Chap. 11) (b) the increase of the enzyme redox potential in order to widen the substrate range (see Chap. 4) (c) the development of heterologous expression and industrial production (see Chap. 12). 

Valderrama B, Ayala M, Vazquez-Duhalt R (2002) Suicide inactivation of peroxidases and the challenge of engineering more robust enzymes. Chem Biol 9 555-565... 

---