Perfusion abdominal aorta

Lung perfusion. Mice are anaesthetized as above, abdomen opened, and the heart exposed. The right ventricle is cut and punctured in the direction of the right atrium with a blunt needle connected to the peristaltic pump. Perfusion is carried out as before, and the abdominal aorta is cut to allow the flow through. After blood clearing and tissue digestion, lungs are removed and placed in a sterile petri dish. 

For immunocytochemical localization of certain amino acid transmitter enzymes in the central nervous system, a good preservation of tissue morphology and antigen antigenicity is essential In general, perfusion fixation is better than immersion fixation. For perfusion fixahon one may perfuse the fixative into the animal circulatory system through the left ventricle of the heart or through the abdominal aorta. 

Fig. 49. Electron diffraction pattern of anatase as shown in Fig. 48. 50 mg of the dust were suspended in 2 ml saline and given intr eritoneally to a 194 g Sprague-Dawley rat (Charles River, France). 4 days later under pentobarbital anaesthesia (30 mg/kg), the animal was perfused from the abdominal aorta with 2.5 % glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4). Postfixation with 1 % osmium tetroxide in sodium cacodylate buffer. Embedded in Epon 812 and sectioned at 50 nm. Stained with lead citrate and uranyl acetate. - Particles analysed were within phagolysosomes of Kupffer cells (block 4460). Film 224/79...

Rats were anaesthetised by intraperitoneal injection of 30 mg pentobarbital/kg and perfused from the abdominal aorta with 2.5 % glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4), postfixed with 1 % osmium tetroxide in sodium cacodylate buffer and embedded in Epon 812. Sections cut at 50 nm were stained with lead citrate and uranyl acetate. 

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