Peptidomics and proteomics

Levy, F Rabel, D., Charlet, M., Bulet, P., Hoffmann, J.A., and Ehret-Sabatier, L. (2004) Peptidomic and proteomic analyses of the systemic immune response of Drosophila. Biochimie 86(9-10), 607-616. 

Biological, individual, and variations between individuals (such as gender, age, and nutrition) affect peptidomes and require careful consideration in order to find valid biomarkers. A few, equally important factors for successful proteomic biomarker research are high sample quality, high sensitivity, and reproducibility that depend on proper selection of the high quality samples. 

FIG U RE 3 STRING protein network evidencing interplay between salivary proteome, peptidome, and proteolytic activities. 

Tammen, H., Schulte, I., Hess, R., Menzel, C., Kellmann, M., Mohring, T., Schulz-Knappe, P. (2005). Peptidomic analysis of human blood specimens comparison between plasma, specimens and serum by differential peptide display. Proteomics 5, 3414-3422. 

The rapid progress in proteomics and peptidomics during the last decade offers us new possibilities to study clinical aspects of disorders and diseases related to the brain [1], These strategies also offer new tools to follow chemical modifications and altered metabolic disturbances that may be indicative of pathophysiological adaptations related to environmental and psychosocial prolonged stress. These techniques can contribute to developments in the diagnostic and therapeutic fields of psychiatric... 

L. Paulson, R. Persson, G. Karlsson, J. Silberring, A. Bierczynska-Krzysik, R. Ekman, and A. Westman-Brinkmalm. Proteomics and Peptidomics in Neuroscience. Experience of Capabilities and Limitations in a Neurochemical Laboratory. J. Mass Spectrometry, 40(2005) 202-213. 

Biosensors and Modern Biospecific Analytical Techniques Analysis and Detection by Capillary Electrophoresis Proteomics and Peptidomics New Technology Platforms Elucidating Biology... 

One major issue underlying salivary peptidome characterization is peptide quantification. While some quantitative methods developed for proteome studies were inherited, peptidome analysis has its own quantification traits. Direct profiling with MALDI-MS or SELDI-MS has been one of the most widely used approaches for peptidome despite its limitations. For instance, Zhang el al. (32,44) used magnetic beads combined with MALDI-MS for saliva profiling in response to orthodontic procedures, solid foods, and teeth... 

Over the recent years, proteomic technologies evolved rapidly and now offer new perspectives in discovery of new cancer biomarkers based on the detection of low molecular weight proteins or peptides by MS. Two main peptidomic approaches are currently under investigation. These include pattern recognition and single/oligo biomarker detection. 

Folkesson, S., Paulson, L, Dahl, A., Rymo, L, Silberring, j., Ekman, R., Blennow, K. (2003). Glinical mass spectrometry in neuroscience Proteomics and peptidomics. Cell Mol. Biol. (Noisy-le-grand) 49, 681-688. 

Ramstrom, M., Bergquist, J. (2004). Miniaturized proteomics and peptidomics using capillary liquid separation and high resolution mass spectrometry, FEES Lett. 567, 92-95. 

Peptidomic analysis of human blood specimens comparison between plasma specimens and serum by differential peptide display. Proteomics 5, 414-422. 

Phytochemistry 62,837-849,2003 Nobeli, I., Ponstingl, H., Krissinel, E.B., and Thornton, J.M., A strncture-based anatomy of the E.coli metabolome, J. Mol. Biol. 334, 697-719, 2003 Parsons, L. and Orban, J., Structural genomics and the metabolome combining computational and NMR methods to identify target hgands, Curr. Opin. Drug Discov. Devel. 7, 62-68, 2004 Soloviev, M. and Finch, P, Peptidomics bridging the gap between proteome and metabolome, Proteomics 6, 744-747, 2006. 

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