Peptides vectors

Rousselle C, Smirnova M, Clair P, Le-fauconnier JM, Chavanieu A, Calas B et al. Enhanced delivery of doxombicin into the brain via a peptide-vector-mediated strategy saturation kinetics and specificity. J Pharmacol Exp Ther 2001 296 124-131. 

Li, J.-M., Collins, L., Zhang, X.-H., Gustafsson, K. and Fabre, J.W. (2000) Efficient gene delivery to vascular smooth muscle cells using a nontoxic, synthetic peptide vector system targeted to membrane integrins a first step toward the gene therapy of chronic rejection. Transplantation, 70, 1616-1624. 

Morris, M.C., Chaloin, L., Mery, I, Heitz, F. and Divita, G. (1999) A novel potent strategy for gene delivery using a single peptide vector as a carrier. Nucleic Acids Res., 27, 3510-3517. 

Rousselle C, Clair P, Lefauconnier JM, et al. New advances in the transport of doxorubicin through the blood-brain barrier by a peptide vector-mediated strategy. Mol Pharmacol 2000 57(4) 679-686. 

The major obstacle to PNA use so far has been poor uptake by most cells and tissues. Therefore, delivery systems based mainly on the use of peptide vectors have been developed. Recent studies, on the other hand, demonstrate that neuronal tissues or cells are an exception to this general rule and spontaneously take up PNA (13-16). The reason for this is not clear, but the fact is that if injected directly into neuronal tissue, or applied to a neuronal cell culture, a delivery vector is not obligatory for cellular uptake of PNA oligomers. 

Schumacher T, Hofer S, Eichhorn K, Wasner M, Zimmerer S, Freitag P, et al. Local injection of the Y-labelled peptidic vector DOTATOC to control gliomas of WHO grades 11 and 111 an extended pilot study. Eur J Nud Med 2002 29 486-493. 

Morris, M.C., P. Vidal, et al. 1997. A new peptide vector for efficient delivery of oligonucleotides into mammalian cells. Nucleic Acids Res 25, 2730-2736. 

A more recent work on the development of a BFC for Ga " is based on a S3N tetra-coordinate chelator (O Fig. 45.8) (Luyt and Katzenellenbogen 2002). It shows high stability at neutral aqueous conditions when coupled to an amino acid mimicking a peptidic vector molecule. This could be interesting for the use with gallium labeled diagnostic radiopharmaceuticals. 

Romer, W., Wu, T.-D., Duchambon, P., et al. (2006) Sub-cellular localisation of a N-labeUed peptide vector using NanoSIMS imaging. Appl SurfSci, 252, 6925-6930. 

Rousselle C, Clair P, Smirnova M, Kolesnikov Y, Pasternak GW Gac-Breton S, Rees AR, Scherrmann JM, Temsamani J Improved brain uptake and pharmacological activity of dalargin using a peptide-vector-mediated strategy. 

Nonviral synthetic vectors (cationic liposomes/polymers, dendrimers, cyclodextrin, ceU-penetrating peptides) Vector can cross cell membrane Easy to handle Technically simple Low transfection efficiency and toxicity in certain cases Yes It is possible to perform site directed injections (microinjections)... 

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