Peptides deuterium exchange

Kamel AM, Zandi KS, Massefski WW. 2003. Identification of the degradation product of ezlopitant, a non-peptidic substance P antagonist receptor, by hydrogen deuterium exchange, electrospray ionization tandem mass spectrometry (ESI/MS/MM) and nuclear magnetic resonance (NMR) spectroscopy. J Pharm Biomed Anal 31 1211. 

In the case of peptide complexes (e.g. 37), only the protons on carbon adjacent to the terminal carboxylate group are acidic enough to allow carbanion formation and to undergo deuterium exchange.65... 

Fig. 7. Top mass spectra of FHV y-peptide after acetylation. The degree and site of acetylations, determined by tandem mass spectrometry, can be used to characterize surface accessible regions of the peptide. Bottom a viral capsid protein ion generated by ESI undergoes hydrogen-deuterium exchange. The multiple populations of ions generated can help distinguish between multiple conformers present for a capsid protein...

Bryan G. Reuben is Professor Emeritus of Chemical Technology at London South Bank University. He has written widely on the technology and economics of the chemical and pharmaceutical industries. His most recent experimental work was on hydrogen-deuterium exchange in protonated peptides and on the downstream processing of nisin. 

Heck, A.J.R. Jorgensen, T.J.D. O Sullivan, M. Von Raumer, M. Derrick, P.J. Gas-Phase Noncovalent Interactions Between Vancomycin-Group Antibiotics and Bacterial Cell-Wall Precursor Peptides Probed by Hydrogen/Deuterium Exchange, J. Am. Soc. Mass Spectrom. 9, 1255-1266 (1998). 

One problem with this approach is that hydrolysis of the peptide can contribute to racemization. Frank et al. developed an approach that involves hydrolysis of the peptide in 6 m DCl in D2O, which leads to deuterium exchange on racemization [49, 50]. The amount of racemization resulting from hydrolysis can then be measured by mass spectrometric determination and subtracted to give a measure of racemization from synthesis of the peptide. 

Pentan-2-amme, 4.4 -(ethyleiiediimino)bis-complexcs structure, 25 Peptides metal complexes deuterium exchange, 426 hydrolysis, 428, 440 Peyrone s reaction, 17 Peyrone s salt configuration, 17 history, 3 Pfeifier, Paul, 1.5 Pfeiffer effect, 26... 

Fig. 11.6. Effect on ferrocytochrome of oxidation at intervals during the time course of exchange of hydrogen for deuterium. The ratio of absorbances of the amide bands,. mideii/ imidci (left ordinate), and the corresponding percent peptide hydrogen exchanged (right ordinate) are plotted against time. The kinetic exchange curves of unmodulated reduced (O—O)...

Gard, E. Grenn, M.K. Bregar, J. Lebrilla, C.B. Gas-phase hydrogen/deuterium exchange as a molecular probe for the interaction of methanol and protonated peptides. J. Am. Soc. Mass Spectrom. 1994, 5, 623-631. 

Kaltashov, I.A. Doroshenko, V.M. Cotter, R. J. Gas phase hydrogen/deuterium exchange reactions of peptide ions in a quadrupole ion trap mass spectrometer. Proteins Struct. Funct. Genet. 1997, 28, 53-58. 

Freitas, M.A. Hendrickson, C.L. Emmett, M.R. Marshall, A.G. High-Field Fourier transform ion cyclotron resonance mass spectrometry for simultaneous trapping and gas phase hydrogen/deuterium exchange of peptides. J. Am. Soc. Mass Spectrom. 1998, 9, 1012-1019. 

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