Peptide coatings

Dalton AB, Ortiz-Acevedo A, Zorbas V, Brunner E, Sampson WM, Collins L, Razal JM, Yoshida MM, Baughman RH, Draper RK, Musselman IH, Jose-Yacaman M, Dieckmann GR (2004) Hierarchical self-assembly of peptide-coated carbon nanotubes. Adv. Funct. Mater. 14 1147-1151. 

Willcox MDP, Hume EBH, Aliwarga Y et al. (2008) A novel cationic-peptide coating for the prevention of microbial colonization on contact lenses. J Appl Microbiol 105 1817-1825... 

The working principle of the ELISA described here involves the use of LHRH peptide-coated wells to capture any anti-LHRH antibodies present in the sera of vaccinated mice. Captured antibodies are detected through the use of a horseradish peroxidase-conjugated antibody that is specific for mouse immunoglobulins and a substrate is then added to induce a color change. In this case, the substrate utilized is 2,2 -azino-bis 3-ethylbenzthiazoline-sulfonic acid (ABTS Sigma-Aldrich, USA), which is converted to a green soluble end product by horseradish peroxidase (see Note 7). The level of substrate-induced... 

Dieckmann et al. in 2003 described an amphiphilic a-helical peptide specifically designed to coat and solubilize CNTs and to control the assembly of the peptide-coated nanotubes into macromolecular structures through peptide-peptide interactions between adjacent peptide-wrapped nanotubes [227]. They claimed that the peptide folds into an amphiphilic a-helix in the presence of carbon nanotubes and disperses them in aqueous solution by noncovalent interactions with the nanotube surface. EM and polarized Raman studies revealed that the peptide-coated nanotubes assemble into fibers with the nanotubes aligned along the fiber axis. The size and morphology of the fibers could be controlled by manipulating the solution conditions that affect peptide-peptide interactions [227]. 

Transfer 50 pL of hybridoma supernatant to the peptide coated plates and incubate 1 h at 37°C. Designate at least 1 well/screen and preferably 1 well/plate as a negative control. In place of hybridoma supernatant, add only DMEM plus 20% FCS to these wells. 

Figure 10 A hypothetical model for the peptide coated CdS crystallite with the cysteinyl thiolates of the peptides providing the surface ligands. The lattice shown is that of a zincblende configuration, but the native particles appear to be more polycrystalline. (Reprinted from Ref 80. 1990 with permission from Elsevier)...

Peptide-coated nanotube-based biosensor Cyclic citrulline peptide ... 

Capture of Cells with Peptide-Coated Magnetic Beads... 

Lim Y-b, Lee E, Lee M (2007) Cell-penetrating-peptide-coated nanoribbons for intracellular nanocarriers. Angew Chem hit Ed 46 3475-3478... 

Figure 9. TEM images of (a) F877 triple helices, (b) Au growth on F877 triple helices, and (c) Au growth on the mineralization peptide-coated triple helices. Scale har=40 nm. Insets ate their HRTEM images. Scale bar = 15 mn.

In this section, we introduce another example of this triple helix peptide coating with oxide semiconductors. This biotemplating method can address both needs for... 

Lagerholm, B.C., Wang, M., Ernst, L.A., Ly, D.H., Liu, H., Bruchez, M.P., and Waggoner, AS. 2004. Multicolor coding of cells with cationic peptide coated quantum dots. NanoLetters... 

Tissue specific Gene delivery Variations in nanoparticle peptide coating density can alter the tissue-specificity of gene delivery in vivo Harris et aL (2010)... 

Nahar K, Absar S, Gupta N, Kotamraju VR, McMurtry IF, Oka M, Komatsu M, Nozik-Grayck E, Ahsan F. Peptide-coated liposomal fasudil enhances site specific vasodilation in pulmonary arterial hypertension. Mol Pharm. Nov 4, 2014. 

Figure 5.112. Atomic force microscopy image of nano-l/SWCNT sample exhibiting a Y-junction (7) and an X-junction (2) (A). Transmission electron microscopy image of peptide coated SWCNTs exhibiting Y-junction apparently created through peptide-peptide interactions provided complementary evidence that individual peptide-wrapped SWCNTs could be isolated using an amphiphilic a-helical peptide (B). (See color insert.) (From Musselman et al. [506], (2004) American Chemical Society used with permission.)...

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