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PCR cloning

DNA Amplification Kit as well as the PCR cloning check. The plasmids with appropriate end-sequences are selected (one clone for one gene) and re-arrayed to their primary positions in a 96-well format. [Pg.34]

Aloe (Aloe arborescens) is a medicinal plant rich in aromatic polyketides such as pharmaceutically important aloenin (a hexaketide pyrone), aloesin (a heptaketide chromone), and barbaloin (an octaketide anthrone) (Fig. 4a). Pentaketide chromone synthase (PCS) and octaketide synthase (OKS) are novel plant-specific type III PKSs, which were obtained from the aloe plant by RT-PCR cloning using degenerate oligonucleotide primers based on the conserved sequences of known CHS enzymes [30-33]. The deduced amino acid sequences of PCS and OKS are 91% identical (368/403), and show 50-60% identity to those of other CHS superfamily type III PKSs of plant origin OKS shares 60% identity (240/403) with CHS from... [Pg.47]

Synthesize a specific oligonucleotide that contains all of the sequence possibilities of the aligned sequences. DNA synthesizers are capable of multiple nucleotide additions at each position, which degenerates the position. Oligos that are as much as 1048-fold degenerate have been used successfully in PCR cloning... [Pg.95]

PCR cloning using genomic DNA as template assumes that introns are not located within the sequence bounded by the PCR primers. This is the case for most chemokine receptors, however several have introns splitting the ORF in the N-terminal region. [Pg.97]

Item Isozyme electrophoresis MCF DNA-DNA hybridization Restriction analysis RAPD Sequencing PCR Cloning... [Pg.15]

Gubili, J. and Borthakur, D. (1996). The use of a PCR cloning and screening strategy to identify lambda clones containing the hupB gene of Anabaena sp. strain PCC 7120. J. Microbiol. Meth. 27,175-182. [Pg.164]

Kern, M.J., Witte, D.P., Valerius, M.T., Aronow, B.J., Potter, S.S. 1992. A novel murine homeobox gene isolated by a tissue specific PCR cloning strategy. Nucleic Acids Res. 20, 5189-5195. [Pg.151]

InsTAclone PCR Cloning Kit (Fermentas Life Sciences K 1214) The kit contains a TA cloning vector (pTZ57R/T), 5x ligation buffer, T4 DNA ligase 5 U/gL, controls and Transform Aid bacterial transformation system with C medium and T-solution A and B. [Pg.403]

All experimental procedures concerning DNA extraction, polymerase chain reaction (PCR), cloning, nucleotide sequencing, molecular phylogenetic analyses, and statistical analysis were conducted as described (Nikoh and Fukatsu, 2000). [Pg.320]

I. Ateitalla et al. (1993). Species-specific induction of cytochrome P-450 4A RNAs PCR cloning of partial guinea-pig, human and mouse CYP4A cDNAs. Biochem. J. 294, 173-180. [Pg.505]

Cai, J. et al., PCR cloning and nucleotide sequence determination of the 18S rRNA genes and internal transcribed spacer 1 of the protozoan parasites Cryptosporidium parvum and Cryptosporidium muris, Biochim. Biophys. Acta 1131, 317, 1992. [Pg.746]

If desired, cDNA of your gene of interest can be easily inserted into pPD129.36 using standard RT-PCR cloning. Inserts greater than 500 base pairs in length tend to be the most effective for RNAi in C. ek ians. [Pg.134]

PCR Cloning Protocols From Molecular Cloning to Genetic Engineering, edited by Bruce A. White, 1996... [Pg.273]

Hang X, Zhang G, Wang G, Zhao X, Chen GQ (2002) PCR cloning of polyhydroxyalkanoate biosynthesis genes from Burkholderia caryophylli and their functional expression in recombinant Escherichia coli. FEMS Microbiol Lett 210 49-54... [Pg.173]


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See also in sourсe #XX -- [ Pg.2 ]

See also in sourсe #XX -- [ Pg.40 ]




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