Pathway for Combinatorial Synthesis and Analysis

There is an enormous wealth of diverse methodologies and methods at our disposal to comprehend and to manage the concept of combinatorial chemistry. In this article however, we wish to focus our discussion on molecules synthesized by organic synthesis. The majority of combinatorial compounds hitherto produced have been synthesized on solid support, either as collections of single compounds or as libraries. The choice between these strategies varies from case to case and depends on individual preferences. In gener- 

In cycle II, the decision must be made as to whether single substances or libraries should be synthesized before these are then submitted to biological screening. Also, for the analysis of the large numbers of compound collections which can be produced after validating the reaction conditions, mass spectrometry is the method of the first choice. However, an aspect which is not considered in this contribution is the use of mass spectrometry for screening purposes [2], 

Compounds generated by the combinatorial approach can be classified according to different aspects. One possible and meaningful attempt is to go for combinatorial diversity (Fig. 17.2) [3] by considering all conceivable organic molecules as a combinatorial space. The latter can be divided into an oligomeric and a monomeric space. It is important 

There is no doubt that for the analysis of all these compounds, mass spectrometry is the first choice to obtain information about the molecular masses of the investigated compounds. Mass spectrometry offers high information content with extremely low sample consumption. In principle, any mass spectrometric method can be employed. From the diverse ionization techniques which are available for the analysis of combinatorially sythesized compounds, especially the ESI-technique but also the MALDI-technique have gained most common acceptance. The reasons for this development are various and are discussed in the following sections. 

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