Pathogens, field detection

O Shea ML, Field R (1992) Detection and disinfection of pathogens in storm-generated flows. Can J Microbiol 38 267-276... 

This section considers, in three separate chapters, the anatomy and physiology of bacteria, fungi and yeasts, and viruses, together with a survey of the characters of individual members of these groups likely to be of importance to the applied field covered by this book. Additional information is provided about more rapid methods for detecting bacteria. The final chapter in this section (Chapter 4) considers the principles of microbial pathogenicity and epidemiology. 

As mentioned in the first section, the use of real-time PCR for high sensitivity detection of relatively limited numbers of analytes is well established in the field thanks in particular to the use of the Ruggedized Advanced Pathogen Identification Device (R.A.P.I.D Idaho Technology, Salt Lake City, UT) by the US military in the last few years. Samples must be manually prepared to separate oligonucleotides from other sample matrix components prior to the analysis, but the amplification of target DNA can be accomplished in 30-60 minutes and the resulting limits of detection are very... 

DNA biosensors have a great potenticd for numerous applications which will include new improvements for the detection of single nucleotide polymorphisms (SNPs) analysis in personedized medicine, pathogenic organisms in the field of food control, and toxic pollutants in environmental monitoring. 

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