Oligonucleotides separation

Proteins, Peptides, Oligonucleotides Separation, Purification Reversed Phase disks [66]... 

Several research groups, for instance, reported on the generation of weak anion exchanges by reacting the epoxy functionalities with diethylamine [54-57], The resulting diethylaminoethyl monoliths—which are commercially available as CIM (Convective Interaction Media) in disk and column format—have frequently been used for protein and oligonucleotide separation [58] as well as for the purihcation of proteins and plasmid DNA [59-61]. 

Dephosphorylated oligonucleotides Polynucleotide kinase + AT p 5 -terminal P-labelled oligonucleotides Separation... 

Figure 10.5.38 Schematic Representation of the Sapphyrin-Functionalized Silica Gel 10.55 Used to Generate Oligonucleotide-separating HPLC Chromatography Columns...

HPLC is commonly used for separating and purifying oligonucleotides. Separation is usually based on ion-pair, reversed-phase chromatography and is particularly useful for purifying fluorescently labeled probes guided by absorbance and fluorescent elution profiles. 

Chemical synthesis of oligonucleotides, separation of trisubstituted pyrophosphates-active dinucleotide derivatives... 

R. BakryandG. K. Bonn, Monolithic poly(glycidyl methacrylatedivinyl-benzene) capillary columns functionalized to strong anion exchangers for nucleotide and oligonucleotide separation,/. Sep. Sci. 16. 2478-2484. 2006. 

Table 3.39 Structural and technical properties of nanobead-agglomerated anion exchangers for oligonucleotide separations.

The latest addition to the family of oligonucleotide separators is the DNA-Swift SAX-IS, which combines a pressure and chemically stable monolithic support coated with functionalized nanobeads. These nanobeads with quaternary ammonium functional groups, optimized for oligonucleotide separations, are similar to those of the DNAPac columns and contribute to the column s high... 

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