PALA

When 6 moles of the substrate analog PALA combine with the enzyme ACTase, two things happen at the same time. The enzyme T unfolds to a more active form R... 

We would like to know the binding energy per mole of PALA and the enthalpy of the transformation T R, but we do not have enough information. Independent studies show that partial unfolding of ACTase occurs on binding of less PALA, in particular, 1.8 mol of PALA cause 43% unfolding and 4.8 mol... 

The tautoraerism of certain difunctional derivatives of l-thia-3,4-diazole has received considerable attention. Pala assigned structure 156 to 2,5-dimercapto-l-thia-3,4-diazole on the basis of infrared spectral data, and Thorn" reached the same conclusion by comparing its ultraviolet spectrum (measured in ethanol) with those of the four possible methylated derivatives. However, the infrared spectrum of a chloroform solution of the parent compound showed bands at 2600-2550 cm indicating an SH group and the probable occurrence of form 157 under these conditions, and this conclusion is supported by the occurrence of SH bands in solid state spectra obtained by Swiss investigators. For a summary of earlier work on these compounds, see reference 187. 

Mormal Italian turpeutlne is the prnduel of Piukj picea and Piintf piK/utfT. Pala/.2o- giv< S ibe following values for a lypical pore commercial sample... 

Pala, G. British Patent 1.016.968 Jan. 12.1966 assigned to Instituto de Angeli. SpA. Italy... 

Gomez Barez, J. A., Garcia ViUanova, R. J., Elvira Garcia, S., Rivas Pala, T., Gonzales Paramas, A. M., and Sanchez, J. (2000). Geographical discrimination of honeys through the employment of sugar patterns and common chemical quality parameters. Eur. Food Res. Technol. 210, 437-444. 

L. Castellani, P. Ferrantelli, M. Sinibaldi, G. Vigliano, Identification of Proteinaceous Adhesives in the Wooden Backing of Piero della Francesca s Painting Pala of Saint Bernardino a Gas Chromatographic Study, Journal of Cultural Heritage, 2, 209 215 (2001). 

The authors gratefully acknowledge Prof. Francesco A. Devillanova for his enthusiasm in stimulating this work, and Dr Annalisa Mancini, Dr Luca Pala, and Dr Alessandro Perra for their kind collaboration. 

Valerio, F., Pala, M., Brescianini, C., Lazaazrotto, A., Balducci, D. (1991) Effect of sunhght and temperature on concentration of pyrene and benzo[a]pyrene adsorbed on airborne particulate. Toxicol. Environ. Chem. 31-32, 113-118. 

Viard B, Amaudrut J, Sala-Pala J, Fakhr A, Mugnier Y, Moise C (1985) J Organomet Chem 292 403... 

Shimizu KT, Pala RA, Fabbri JD, Brongersma ML, Melosh NA (2006) Probing molecular junctions using surface plasmon resonance spectroscopy. Nano Lett 6 2797-2803... 

Parodi, S., M.Taningher, P.Russo, M.Pala, M.Tamaro, and C.Monti-Bragadin. 1982. DNA damage in liver, kidney, bone marrow and spleen of rats and mice treated with commercial and purified aniline as determined by alkaline elution assay and sister chromatid exchange induction. Cancer Res. 42 2277—2283. 

In practice, these considerations usually reduce A G si,- i er by > three orders of magnitude (> — 4 kcal mol-1) since observed effective concentrations > 105 M are rarely if ever seen in bisubstrate inhibitors. For example, N-phosphonoacetyl-L-aspartate (PALA),1241 in clinical trials in the 1980s, is a typical bisubstrate analog1251 with Kt = 27 nM (Fig. 11) and an observed effective concentration of > 17 M (equa-... 

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