Packed Column Characteristics

FIG. 14-47 Efficiency characteristics of packed columns (total-reflux distillation.)... 

In a similar manner to the design process for packed columns, the physical characteristics and the performance specifications can be calculated theoretically for open tubular columns. The same protocol will be observed and again, the procedure involves the use of a number of equations that have been previously derived and/or discussed. However, it will be seen that as a result of the geometric simplicity of the open tubular column, there are no packing factors and no multi-path term and so the equations that result are far less complex and easier to manipulate and to understand. 

The design process for open tubular columns is similar to that for packed columns, and the physical characteristics and performance specifications can be calculated... 

The performance and basic characteristics of the packed column can be checked by running the test chromatograms as follows ... 

This paper will be limited to a discussion of our packed column studies in which we have addressed attention to questions regarding, (a) the role of ionic strength and surfactant effects on both HDC and porous packed column behavior, (b) the effects of pore size and pore size distribution on resolution, and (c) the effects of the light scattering characteristics of polystyrene on signal resolution and particle size distribution determination. 

Relationship Between Colunn Diameter and Flow Characteristics in Packed Column Liquid Chromatograidiy... 71... 

Relationship Between Column Diameter and Characteristics in Packed Column Llguld Chromatography... 

Current commercial silica-based columns have two important characteristics (1) they can produce efficiency similar to that of columns packed with 3.5 /tm particles and (2) they typically produce a pressure drop of half that caused by a column packed with 5 /tm particles.35 Monolithic columns have been shown to exhibit flat van Deemter curves, resulting in little loss of efficiency at high flow rates.36 As a result, high-throughput separations on conventional HPLC instruments can be achieved by increasing flow rate up to nine times (up to 9 ml/min) the usual rate in a conventional packed column. Cycle times for HPLC analysis as short as 1 min (injection-to-injection) have been reported by users of monolithic columns. Additional benefits of monolithic columns cited include... 

Preparative chromatographic processes are of increasing importance particularly in the production of fine chemicals. A mixture of compounds is introduced into the liquid mobile phase, and this then flows through a packed column containing the stationary solid phase. The contacting scheme is thus differential, but since the adsorption characteristics of the compounds in the mixture are similar, many equivalent theoretical stages are required for their separation. Chromatographic processes are mostly ran under transient conditions, such that... 

CEC is often inappropriately presented as a hybrid method that combines the capillary column format and electroosmotic flow employed in high-performance capillary electrophoresis with the use of a solid stationary phase and a separation mechanism, based on specific interactions of solutes with the stationary phase, characteristic of HPLC. Therefore CEC is most commonly implemented by means typical of both HPLC (packed columns) and CE (use of electrophoretic instrumentation). To date, both columns and instrumentation developed specifically for CEC remain scarce. 

The measurement of effluent volume is not very reliable because of the effect of the geometry and packing characteristics of any column. It is often more useful to use a reduced parameter, such as V/V0, which is not so dependent upon column characteristics and is comparable with the calculation for RF values in thin-layer chromatography. 

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