P ligand binding

Grubmiiller et al., 1996] Grubmiiller, H., Heymann, B., and Tavan, P. Ligand binding and molecular mechanics calculation of the streptavidin-biotin rupture force. Science. 271 (1996) 997-999... 

Ultraviolet and visible Absorption of UV and visible radiation leading to electronic excitation N, P Ligand binding sites, side chain exposure, environment. Apphcable to proteins and nucleic acids but not glycans. Quantitative analysis. DNA conformation. Anneahng and hybridization studies of nucleic acids. 

Wight C A and Armentrout P B 1993 Laser photoionization probes of ligand-binding effects in multiphoton dissociation of gas-phase transition-metal complexes ACS Symposium Series 530 61-74... 

The oxygen affinity of the derivative was shown to be about half that of unmodified hemoglobin under similar conditions, but a degree of cooperativity was preserved. Kquilihrium and kinetic ligand-binding studies on this derivative have been interpreted (62) to show a perturbed R state. It is beheved that although the reaction is between the two P-chains, aP-dimers function independentiy, probably through a flexible connection. 

P-site ligands inhibit adenylyl cyclases by a noncompetitive, dead-end- (post-transition-state) mechanism (cf. Fig. 6). Typically this is observed when reactions are conducted with Mn2+ or Mg2+ on forskolin- or hormone-activated adenylyl cyclases. However, under- some circumstances, uncompetitive inhibition has been noted. This is typically observed with enzyme that has been stably activated with GTPyS, with Mg2+ as cation. That this is the mechanism of P-site inhibition was most clearly demonstrated with expressed chimeric adenylyl cyclase studied by the reverse reaction. Under these conditions, inhibition by 2 -d-3 -AMP was competitive with cAMP. That is, the P-site is not a site per se, but rather an enzyme configuration and these ligands bind to the post-transition-state configuration from which product has left, but before the enzyme cycles to accept new substrate. Consequently, as post-transition-state inhibitors, P-site ligands are remarkably potent and specific inhibitors of adenylyl cyclases and have been used in many studies of tissue and cell function to suppress cAMP formation. 

Structural aspects and coordination chemistry of metal porphyrin complexes with emphasis on axial ligand binding to carbon donors and mono- and di-atomic nitrogen and oxygen donors. P. D. Smith, B. R. James and D. H. Dolphin, Coord. Chem. Rev., 1981,39, 31-75 (170). 

Reddy MR, Erion MD, Agarwal A. Free energy calculations Use and limitations in predicting ligand binding affinities. In Lipkowitz KB, Boyd DB, editors. Reviews in Computational Chemistry, Vol. 16. New York Wiley-VCH, 2000. p. 217-304. 

Lowry, O.H. Rosebrough, N.J. Farr, A.L. and Randall, R.J. Protein measurement with folin phenol reagent. J Biol Chem 193 265-275, 1951. Munson, P.J.. and Rodbard, D. LIGAND A versatile computerized approach for characterization of ligand binding systems. Anal Biochem 107 220-237. 1980. 

Munson, P., and Rodbard, D. Ligand A versatile approach for characterization of ligand-binding systems. Anal Biochem 107 220-239, 1980. 

Lobel D., Marchese S., Krieger J., Pelosi P., et al. (1998). Subtypes of odorant-binding proteins heterologous expression and ligand binding. Europ J Biochem 254, 318-324. 

P11 ap11, C. Contreras, P.C. O Donohue, T.L and Quirion, R. Autoradiographic distribution of (3H)dexoxadrol, a phencyclidine-related ligand, binding sites in rat and human brain. Neurosci Lett (in press). 

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