Ligand binding studies

The oxygen affinity of the derivative was shown to be about half that of unmodified hemoglobin under similar conditions, but a degree of cooperativity was preserved. Kquilihrium and kinetic ligand-binding studies on this derivative have been interpreted (62) to show a perturbed R state. It is beheved that although the reaction is between the two P-chains, aP-dimers function independentiy, probably through a flexible connection. 

Compared with the conventions adopted for discussing the relationship between drug concentration and response (Chapter 1), a rather different terminology has evolved for ligand-binding studies. 

Four kinds of ligand-binding studies will be discussed (1) saturation, (2) kinetic, (3) competition, and (4) retardation. 

In a ligand-binding study, the measured binding includes AR as well as AR. The relevant equation then is ... 

Measurement of brain neurochemical features by means of PET ligand binding studies or MRI spectroscopy (MRS), which can quantify chemicals of high concentration in the brain. 

Q. Hong, J.-L. Popot, J. H. Lakey (2003) Stabilising a membrane protein with amphipols for surface plasmon resonance ligand binding studies. In preparation... 

The above discussion provides only a brief overview of how fluorescence techniques can be used to study the interactions of ligands with their receptors. We have focused on the quantitation of the binding parameters and compared the data with that which may be obtained with those from radiolabelled ligand binding studies. The number of applications of fluorescence in the study of neurochemistry and molecular biology is ever increasing. Outside the scope of this review is, for example, the use of fluorescence microscopy to monitor cell surface expression and targeting of receptors or the use of fluorescence probes to monitor ion transport into and out of cells. 

Radioiabeiied Ligand Binding Studies Separation Of Free From Bound Ligand.266... 

Rovati, G. E. Ligand-binding studies old beliefs and new strategies. Trends Pharmacol. Sci. 1998, 19, 365—369. 

Smith, D.L. Dharmasiri, K. Protein-ligand binding studied by amide hydrogen exchange and mass spectrometry. NATO ASI Ser. C 1998, 510, 45-58. 

Marquardsen, T., et al., Development of a dual cell, flow-injection sample holder and NMR probe for comparative ligand-binding studies. JMagn Reson, 2006, 182, 55-65. 

Term used to describe quantitatively the separation of a ligand from a receptor. In ligand binding studies it may be expressed as the reciprocal of the affinity constant. 

Lartigue A., Campanacci V., Roussel A., Larsson A. M. and Alwyn T. (2002a) X-ray structure and ligand binding study of a moth chemosensory protein. J. Biol. Chem. 277, 32094-32098. 

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