P-Adrenergica

Pre-treatment of human bronchial epithelial cells with 10 [jM isoprenaline or 100 juM salbutamol augmented the adhesion of fluorescently labelled THP-1 cells, a monocyte/macrophage cell line, to human bronchial epithelial cell monolayers by about 40-60 % (Romberger et al. 2(XX)). The increase in THP-1 cell adhesion occurred within 10 min of isoprenaline pre-treatment of human bronchial epithe-Ual cells and gradually decUned but persisted with up to 24 h of isoprenaUne exposure. 

30 min after an inhalation of 300 fig micronized (mass median diameter 2.45 fim) phenylephrine bitartrate/puff from a suspension type self-propelled aerosol (Medihaler). 12 Puffs/min were dispersed into a 164.5 1 box where the animals stayed for 15 min. 30 min later under methitural anaesthesia, the lung was fixed by intratracheal instillation of 2.5 % glutaraldehyde in phosphate buffer (pH 7.4) before opening the thorax. Postfix-ation with 1 % osmium tetroxide in phosphate buffer (pH 7.4). Contrasted en bloc for 12 h with 0.5 % uranyl acetate in 70 % ethanol. Embedded in a 2 8 mixture of methyl and butyl methacrylate. Sectioned at 50 nm. Lead citrate after Reynolds (1963). Plate 44/05 

5 mM Isoprenaline added after two hours of incubation had no effect on the secretion of Clara cell secretory protein by lung explants (Hook et al. 1990). 

Isoprenahne increased mitotic index and goblet cell number at most of the airway levels studied in the Sprague-Dawley rat (Bolduc and Reid 1978). 

In the pig, isoprenaline caused a significant increase in the number of goblet cells in the bronchial epithelium, but there was no change in their glycoprotein type, most containing exclusively sialomu-cin (Baskerville 1976). 

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