Oxidatively damaged cells

Lee et al., 2001 Murine osteoblastic MC3T3 cells Genistein at physiological concentrations stimulates cell proliferation and prevents oxidative damage. 

Both thermodynamic and kinetic factors are of importance for antioxidant capacity. The antioxidant has to be located in the right position at the right time in order to prevent oxidative damage to vital cell components and will need to be regenerated from the one-electron oxidised form in a recycling process ... 

Lowe, G.M. et al.. Lycopene and beta-carotene protect against oxidative damage in HT29 cells at low concentrations but rapidly lose this capacity at higher doses. Free Rad. Res., 30, 141, 1999. 

The accumulation of hydroperoxides and their subsequent decomposition to alkoxyl and peroxyl radicals can accelerate the chain reaction of polyunsaturated fatty-acid p>eroxidation leading to oxidative damage to cells and membranes as well as lipoproteins. It is well-recognized that transition metals or haem proteins, through their... 

The well-known fact that in irreversibly damaged cells, respiratory control is lost and is accompanied by oxidation of cytochromes a and as, as well as NADH (Taegtmeyer et al., 1985), was originally thoug it to be due to substrate deficiency (Chance and Williams, 1955) but may be due to an enzymatic defect resulting in an inability to metabolize NADH-linked substrates (Pelican etal., 1987). It seems likely therefore that return of function is dependent on preservation of mitochondrial membrane integrity, and the structure and activities of respiratory chain (R.C) complexes I-IV (Chance and Williams, 1955). 

Production of Mucosal Damage 2.3.1.2.1 Cell culture Stimulated neutrophils are known to be cytotoxic to cells in vitro (Dull et al., 1987 Dallegri et al., 1990 Grisham et al., 1990b). Several in vitro systems have been used to demonstrate oxidative damage to intestinal cells. Xanthine/XO increased Cr release and decreased [ H]thymidine uptake by IEC-18 small intestinal epithelial cell monolayers in a dose-dependent manner (Ma et al., 1991). Rat enterocytes show decreased trypan blue exclusion and increased protein release when incubated with neutrophils stimulated... 

Aminosalicylate oxidation by activated leukocytes and protection of cultured cells from oxidative damage. Biochem. Pharm. 36, 2467-2472. 

M. (1982). Auto-oxidative damage in Beh het s disease-endothelial cell damage following the elevated oxygen radicals generated by stimulated neutrophils. Clin. Exp. Imunol. 49, 247-255. 

Zimmerman, RJ., Chan, A. and Leadon, S.A. (1989). Oxidative damage in murine tumour cells treated in vitro by recombinant human tumour necrosis factor. Cancer Res. 49, 1644-1648. 

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