Oxidative stress osteoblasts

Very little work has been reported on the role of oxidative stress in osteoblasts. However, osteoblasts can be induced to produce intracellular ROS (Cortizo et al., 2000 Liu et al., 1999), which can cause a decrease in alkalinephosphatase (ALP) activity that is partially inhibited by vitamin E and cause cell death (Cortizo et al., 2000 Liu et al., 1999). Treatment of rat osteosarcoma ROS 17/2.8 cells with tumor necrosis factor-alpha (TNF-a) suppressed bone sialoprotein (BSP) gene transcription through a tyrosine kinase-dependent pathway that generates ROS (Samoto et al., 2002). H202 modulated intracellular calcium (Ca2+) activity in osteoblasts by increasing Ca2+ release from the intracellular Ca2+ stores (Nam et al., 2002). 

Cortizo, A.M., Bruzzone, L., Molinuevo, S., and Etcheverry, S.B. 2000. A possible role of oxidative stress in the vanadium-induced cytotoxicity in the MC3T3E1 osteoblast and UMR106 osteosarcoma cell lines. Toxicology 147, 89-99. 

Molinuevo, M.S., D.A. Barrio, A.M. Cortizo, and S.B. Etcheverry. 2004. Antitumoral properties of two new vanadyl(IV) complexes in osteoblasts in culture Role of apoptosis and oxidative stress. Can. Chemo. Pharm. 53 163-172. 

In experiments with cultured cells it has been shown that osteocytes, but not periosteal fibroblasts, are extremely sensitive to fluid flow, resulting in increased prostaglandin as well as nitric oxide production [104, 105], Three different cell populations, namely osteocytes, osteoblasts, and periosteal fibroblasts, were subjected to two stress regimes, pulsatile fluid flow and intermittent hydrostatic compression [104], Intermittent hydrostatic compression was applied at 0.3 Hz with a 13-kPa peak pressure. The pulsatile fluid flow was a fluid flow with a mean shear stress of 0.5 Pa with cyclic variations of 0.02 Pa at 5 Hz. The maximal hydrostatic pressure rate was 130 kPa/sec and the maximal fluid shear stress rate was 12 Pa/sec. Under both stress regimes, osteocytes appeared more sensitive than osteoblasts, and osteoblasts more sensitive than periosteal fibroblasts. However, despite the large difference in peak stress and peak stress rate, pulsatile fluid flow was more effective than intermittent hydrostatic compression. Osteocytes, but not the other cell types, responded to 1 hour pulsatile fluid flow treatment with a sustained prostaglandin E2 upregula-... 

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