Other biological materials and biologically active substances

5 Other biological materials and biologically active substances 

The reports published so far clearly indicate that the prevalent applications of gel chromatography are those on proteins. In addition to the latter, GPC has been employed in studies on a number of other biological materials and substances with various biological activities (hormones, vitamins, antibiotics and others). Only a few examples are presented. 

A comparison of various methods for the study of noradrenaline fixation to various proteins showed that the most useful results were obtained by gel chromatography on Sephadex G-25 [147]. Croizer et al. [148] included GPC in the purification process for plant hormones (gibberelins, indole-3-acetic acid, abscisic acid). Chromatography was carried out on Sephadex columns in the environment of tetrahydrofuran (THF) or a mixture of 100 mM acetic acid in THF. The efficient partial purification of the plant extracts thus achieved allowed the application of high resolution procedures. 

Gel chromatography on Sephadex LH-20 offers good separation of biologically active forms of vitamin A both in standard solutions and in biological materials [153]. Holasova and Blattn [154] studied specific binding of an active form of vitamin A, retinol, to retinol receptors. 

GPC has also been employed in determining herbicides in plant [155] and animal [155,156] tissues. 

---