Ornithin Hydrochlorid

The reaction mixture contained in a final volume of 1 mL 20 mAf sodium phosphate buffer (pH 5.0), 2.5 mAf dithiothreitol, 0.1 mAf pyridoxal 5 -phosphate, 0.4 /xCi L-[2,3-3H]ornithine, 100 mAf ornithine hydrochloride, and 0.3 mL of either E. coli ornithine decarboxylase or tissue homogenate. The E. coli enzyme was assayed at pH 5.0 and 37°C the pH values of reaction mixtures were adjusted to pH 7.3 before assaying homogenates of mammalian tissues. The reaction was stopped by injection onto the column. 

N -DNP-N -bromoacetyl-h-omithine (BADO). N -DNP-L-ornithine hydrochloride (1.68 g, 5 mmoles) is dissolved in cold water containing 2 equivalents of NaOH. To this solution, 3 equivalents of BrCHsCOBr are added and the reaction is allowed to proceed at 0° for 20 min, during which the pH is maintained above 8 with NaHCOs. The reaction is allowed to continue for another 10 min at room temperature, after which the mixture is acidified to pH 2 with HCl and the product is extracted into ethyl acetate. The extract is washed with water, dried over anhydrous Na2S04, and concentrated to dryness. After recrystallization from ethyl acetate-petroleum ether the yield is 1.7 g (80%) m.p. 157°-159°. 

Anonymous. Eflornithine hydrochloride. d,l-alpha-Difluoromethyl ornithine. DFMO. Drugs Future 1986 11 220. 

N-iminoethyl-L-ornithine l-NIO. minourea hydrochloride guanidine, mipemide imipenem. 

Gramicidin contains no acidic or basic groups. Tyrocidine contains strongly basic groups due to ornithine residues. It can be isolated only as the hydrochloride. The crystalline mixture tyrocidine contains 1-omithine, 1-proUne, 1-valine, 1-leucine, d-phenylalanihe, 1-tryptophane, 1-tyrosine, 1-aspartic acid and 1-glutamic acid (11). 

D-193 DL-a-Difluoromediylomithine hydrochloride Irreversible inhibitor of ornithine decarboxylate (ODC) chemoprotective agent that blocks angiogenesis. 

The cream contains 13.9% (139 mg/g) anhydrous eflomi-thine hydrochloride as eflornithine hydrochloride monohydrate (150 mg/g). Chemically, eflornithine hydrochloride is ( )-2-(difluoromethyl)ornithine monohydrochloride monohydrate, with the empirical formula C6H12F2N202 ... 

Incorporation of a suitably protected ornithine followed by derivati-zation with guanylating agents such as 1-guanylpyrazole hydrochloride to form arginine is an alternative method of incorporating Arg residues in a peptide [194,195]. This has been applied to both Boc and Fmoc SPPS. How-... 

Isopentane Neopentane n-Pentane C5H12CINO2 Betaine hydrochloride C5H12NO3PS2 Dimethoate C5H12NO4PS Omethoate C5Hi2N20 N-Butylurea Tetramethylurea C5H12N2O2 Ornithine C5H12N2S 1,3-Diethylthiourea C5H12O n-Amyl alcohol s-Amyl alcohol t-Amyl alcohol... 

Fig. 7. Acetylornithinase formation from accumulated mRNA in the orgR strain 961 A) and the argR" strain 977 (5) of Escherichia coii. After accumulation of mRNA by arginine starvation at 37° for 30 minutes, rifampicin (60 jug/ml) is added and allowed to act for 2 minutes. L-Ornithine (open circles), or L-arginine (solid circles), is added (as hydrochlorides, at 0.1 mg/ml) for translation at 37° the addition corresponds to 0 time on the abscissa. From McLellan and Vogel [68].

Whereas L-citrulline hydrochloride (4) is, like arginine, characterized by three planar groups which in this case are (a) the carboxyl group, (b) the side-chain, and (c) the urea function, with dihedral angles a b 91° and b c 87°, DL-ornithine hydrobromide (5) and Lay-diaminobutyric acid monohydrochloride (6) may be more simply represented by the two planes of... 

This correlation should also be applicable to the hydrochlorides of NH2" type acids, but, as in previous cases, not much information is available. Sarcosine hydrochloride again appears to be slightly abnormal in showing its carbonyl absorption at 1757 cm", but both proUne and A-phenylglycine hydrochloride absorb at 1730 cm". Histidine hydrochloride absorbs at 1706 cm" and ornithine dihydrochloride [13] at 1739 cm". As far as the limited data available allow, therefore, it seems reasonable to assume that the correlation is obeyed by this type of compound. 

Methyl y - cyano -a- D - carbobenzoxy-aminobutyrate hydrogenated 2 hrs. with Raney-Ni in acetic acid at 100° and an initial pressure of 90 atm., the product hydrolyzed with 3 iV HCl and isolated as the hydrochloride D-ornithine hydrochcloride. Y 63%. (1963). 

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