Proteomics organelle

Brunet, S., Thibault, P., Gagnon, E., Kearney, P, Bergeron, J.J., Desjardins, M. (2003). Organelle proteomics looking at less to see more. Trends. Cell. Biol. 13, 629-638. 

Gene Therapy Protocols Volume 1 Production and In Vivo Applications of Gene Transfer Vectors, edited by Joseph M. LeDoux, 2008 432. Organelle Proteomics, edited by Delphine Pflieger and Jean Rossier, 2008 431. Bacterial Pathogenesis Methods and Protocols, edited by Prank DeLeo and Michael Otto, 2008... 

Studies of Drug Resistance Using Organelle Proteomics... 

ORGANELLE PROTEOME VARIATION AMONG DIFFERENT CELL TYPES LESSONS FROM NUCLEAR MEMBRANE PROTEINS... 

Organelle Proteome Variation Among Different Cell Types ... 

Dunkley TP, Hester S, Shadforth IP, Runions 1, Weimar T, Hanton SL, Griffin JL, Bessant C, Brandizzi F, Hawes C, Watson RB, Dupree P, Lilley KS. Mapping the Arabidopsis organelle proteome. Proc. Natl. Acad. Sci. U.S.A. 2006 103 6518-6523. 

Subcellular fractionation, sucrose density gradients Allows isolation of specific cellular organelles for organelle proteome analysis difficult to obtain completely pure preparations... 

Proteomics research has benefited greatly from subcellular fractionation, because reducing the complexity of the entire proteome to smaller organelle proteomes makes it possible to separate and detect low abundance proteins. Furthermore, since the goal of proteomics is not only to learn protein sequences but also the locahzation and function of proteins, subcellular analysis is advantageous because it provides the subcellular localization. Indeed, the benefits of cellular fractionation before... 

The Isotope Coded Affinity Tags method is used to compare and quantify relative protein levels between different samples. Proteins of one sample are labeled with a heavy, the other with a light isotope of the reagent. The samples are combined, digested and used in a first roimd of MS to quantify the relative abimdances of each peptide pair (heavy and light). Subsequent fragmentation determines the peptide amino-acid sequences. Dunkley et al. [122] successfully applied ICAT for organelle proteome studies in Arabidopsis. 

Hu, Z.Z., Valencia, J.C., Huang, H. et al.. Comparative bioinformatics analyses and profiling of lysosome-related organelle proteomes. Int. J. Mass Spectrom. (2006), in press. 

---