Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Optical arrangements for single molecule detection

Next we shall describe these two common experimental geometries, but before we proceed we re-emphasize the key considerations of the experiment to maximize the signal-to-noise  [Pg.102]

Clearly, single-photon far-field excitation in this manner provides no spatial reduction of the excitation/collection volume in the direction of propagation of the light and so additional optics are required in the detection path to minimize [Pg.104]

One-and two-photon epi-fluorescence permit similar experiments to be carried out, although one may have practical advantages over the other [19]. [Pg.108]

2 The PSF in single-photon confocal and two-photon epi-fluorescence illumination systems [Pg.108]

In confocal microscopy the PSF is a function of a number of physical components, namely the laser beam, the microscope objective, the confocal pinhole, and the detector. The PSF is a convolution of the effect of all of these components. Despite this apparent complexity it is perhaps surprising that a simple description of the PSF for these microscopes has been widely applied in which the sample volume is described by a three-dimensional Gaussian with a 1/e beam waist diameter 2coq and a length 2zq along the optic axis [6,20,21 ] and is given by, [Pg.109]


Figure 9. (a) Diagram of the hyperbolic electromagnetic trap for levitating microdroplets in a laser beam. (b) Optical arrangement for single-molecule detection in microdroplets lens, L beam splitter, BS polarizer, P trap, T microscope objective, O optical filters, F spatial filter, SF photomultiplier, PMT desiccant chamber, D. (Adopted from [51].)... [Pg.15]


See other pages where Optical arrangements for single molecule detection is mentioned: [Pg.9]    [Pg.102]   
See also in sourсe #XX -- [ Pg.102 , Pg.103 , Pg.104 , Pg.105 , Pg.106 , Pg.107 , Pg.108 , Pg.109 , Pg.110 , Pg.111 , Pg.112 , Pg.113 , Pg.114 , Pg.115 , Pg.116 , Pg.117 , Pg.118 ]




SEARCH



Molecule detection

Optical arrangements

Optical arrangements, for single molecule

Optical detection

© 2024 chempedia.info