Open-tubular liquid chromatography

Tan, ZJ Remcho, VT, Molecular Imprint Polymers as Highly Selective Stationary Phases for Open Tubular Liquid Chromatography and Capillary Elechophoresis, Elechophoresis 19, 2055, 1998. 

OTLC Open-tubular liquid chromatography PIL Plasma-induced luminescence... 

Fujimoto, C., Sakurai, M., and Muranaka, Y. (1999). PEEK columns for open-tubular liquid chromatography with electroosmotic flow. /. Microcolumn Sep. 11, 693-700. 

Kennedy, R. T. and Jorgenson, J. W., Quantitative-analysis of individual neurons by open tubular liquid-chromatography with voltammetric detection. Analytical Chemistry 61(5), 436 441, 1989. 

Figure 15. Open tubular liquid chromatography of amine-NBD derivatives using on-column fluorescence detection. Peaks correspond from left to right to ethylamine, n-propylamine, n-butylamine, cyclohexylamine, and n-hexylamine. Conditions 20-p.m X 8.3-m column with C-18 bonded phase 20% acetonitrile and 80% water (v/v) mobile phase at a linear velocity of 0.50 cm/s on-column injection of 5 nL. (Reproduced from reference 59. Copyright 1984 American Chemical Society.)...

Optical absorbance detection was also achieved using a CCD-based spectrophotometer. Two food dyes (FD C Blue 1 dye and FD C Red 3 dye) were first separated by open-tubular liquid chromatography and then detected on a PDMS chip. The LODs for the blue and red dyes were determined to be 80 pM and 200 pM, respectively [569]. 

Especially in open tubular liquid chromatography (OTLC) the solubility of the sample components in the mobile phase rather than in the stationary phase may be the limiting factor. 

The same group developed an open-tubular liquid chromatography (OTLC) column by chemically binding BSA to the inner surface of a fused-silica capillary [222]. A number of enantioseparations have been presented, including DNP-amino acids and 3-hydroxy-1,4-benzodiazepines. 

The process of band broadening (Figure 2.1) is measured by the column efficiency or the number of theoretical plates N, equation (2.24)), which is equal to the square of the ratio of the retention time to the standard deviation of the peak. In theory, the value of N for packed columns has only a small dependency on k and may be considered to be a constant for a particular column. Column efficiency in open-tubular systems decreases markedly with increased retention. For this reason open-tubular liquid chromatography systems must be operated at relatively low kf values (see section 2.5.S.2). 

Manz, A. and Simon, W. (1986) Potentiometer detector for fast high-performance open-tubular liquid chromatography. Anal. Chem. 59, 74 79. 

W. M.A. Niessen and H. Poppe, Open-tubular liquid chromatography-mass spectrometry with a capillary-inlet interface, J. Chromatogr., 385 (1987) 1. 

De Witt, J.S.M., C.E. Parker, K.B. Tomer, and J.W. Jorgenson. 1988. Separation and identification of trifluralin metabolites by open-tubular liquid chromatography/negative chemical ionization mass spectrometry. Biomed. Environ. Mass Spectrom. 17 47-53. 

Swart, R., Brouwer, S., Kraak, J. C., Poppe, H., Swelling behaviour and kinetic performance of polyacrylate stationary phases for reversed-phase and normal phase open-tubular liquid chromatography. /. Chromatogr. A1996, 732, 201-207. 

Knox, J. H., Gilbert, M. T, Kinetic optimization of straight open-tubular liquid chromatography. J. Chromatogr. 1979,186, 405-118. 

Recent progress in open tubular liquid chromatography. Trends Anal. Chem. 1997,16, 332-342. 

The wire electrode is advantageous when miniaturization is necessary. Knecht et al. (1984) have described the use of single carbon fibers (diameter < 10/working electrode was plac directly into the outlet of the column by means of a microscope. The rest of the detector cell was situated outside the tube (Fig. 25). 

Tan, Z.J. Remcho, V.T. Molecular imprint polymers as highly selective stationary phases for open tubular liquid chromatography and capillary electrochromatography. Electrophoresis 1998,19, 2055-2060. 

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