Oligosaccharides from cellulose

The Polymer-homologous Series of Oligosaccharides from Cellulose, M. L. Wolfrom and J. C. Dacons, J. Amer. Chem. Soc., 74, 5331-5333 (1952). 

For monitoring the extent of polysaccharide hydrolysis, l.c. methods that sepeu ate and analyze the non-fermentable oligosaccharides (d.p. 3-30) derived from cellulose, hemicellulose, and pectins are useful, and have already been described (see Section III,l,c). For determination of the monosaccharide composition of completely hydrolyzed, plant polysaccharides, l.c. is especially useful and has been applied to the compositional analysis of hydrolyzed plant fiber,wood pulps,plant cell-walls,and cotton fibers.In these representative examples, the major sugars of interest, namely, glucose, xylose, galactose, arabinose, and mannose, have traditionally been difficult to resolve by l.c. The separa-... 

Sephadex G-25 water oligosaccharides from acetolysis of cellulose 82... 

Unlike starch or cellulose, BSG hemicellulose has a complex structure, which is still present, in part, on its autohydrolysis products. Oligosaccharides from BSG hydrolysis consist mainly of branched arabino-xylo-glucurono oligosaccharides that are not highly acetylated when compared to other xylans, such as from Eucalyptus wood (31). The action of several enzymatic activities including endo-l,4-P-xylanase P-xylosidase and accessory activities such as acetyl xylanesterase, a-glucuronidase, and a-arabino-furanosidase is therefore required for the complete hydrolysis of OCL to monosaccharides. 

FIGURE 10.13 Microchip electropherograms of 8-aminopyrene-l,3,6-trisulfonate (APTS)-labeled N-linked oligosaccharides from ribonuclease B (A) and APTS-labeled oligosaccharide ladder (B). Experimental conditions Esep = 300 V/cm, 0.5% methyl cellulose in 20 ruM phosphate buffer (pH 6.66). (Reprinted from Dang, R, Journal of Chromatography A 1109, 141, 2006. With permission.)... 

R 126 M. A. Kabel, H. A. Schols and A.G.J. Voragen, Identification of Structural Features of Various (O-Acetylated) Xylo-Oligosaccharides from Xylan-Rich Agricultural By-Products A Review , p. 108 R 127 P.T. Larsson, Interaction between Cellulose I and Hemicelluloses Studied by Spectral Fitting of CP/MAS C-NMR Spectra , p. 254 Vol. 867, 2004... 

The n.m.r. spectra of peracetylated cello-oligosaccharides have been recorded and compared with that of cellulose acetate.All of the signals from cellulose acetate were only observed in the spectra of cellotetraose and cello-pentaose peracetates. Some signals were not observed in the spectra of cellobiose and cellotriose peracetates. 

Besides the many known naturally occurring free oligosaccharides, a great variety of this class of compound can be obtained by enzymic degradation or controlled hydrolysis of a polysaccharide with acid. As an example, the treatment of starch with amylases produces maltose under certain conditions of acid hydrolysis, isomaltose can be obtained from starch, and cellobiose from cellulose. 

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