In neoglycoprotein preparation

In the preparation of affinity adsorbents, it is necessary to attach the ligand residues to insoluble supports by chemical bonds or physical interactions. Adsorbents having carbohydrate ligands have been prepared for the most part by chemical reaction of carbohydrate derivatives with the support material. Methods for the preparation of some types of carbohydrate derivatives are described in Section IV. Methods for the preparation of other derivatives of carbohydrates that may be suitable for synthesizing affinity adsorbents have been described in an article on neoglycoproteins.31... 

A transglutaminase reaction was found to be useful in the preparation of neoglycoproteins. For example, oo-amino glycosides were used as a substrate for transglutaminase for the addition of carbohydrates to glutamine side chains (35, 36). The success of this reaction, however, seems somewhat unpredictable. 

The advancement in the techniques of the chemical synthesis of oligosaccharides and peptides (including molecular engineering) elevated the neoglycoprotein preparation to the de novo synthesis level, which opened a wide range of application possibilities. 

Compare to historical ill-defined neoglycoprotein, major progresses have been made in the preparation of well defined multivalent structures based on dendrimeric scaffold. Among the building blocks used for such construction, carbohydrate-based blocks allow an access to a versatile panel of chiral and branched scaffolds. The major advantages of carbohydrate cores are ... 

Because O-glycosylation can also be accomplished with active esters (e.g., penta-fluorophenyl esters) [11] of Fmoc serine and threonine, the Fmoc technique provides a general method for the synthesis of glycopeptides. Thomsen-Friedenreich antigen glycopeptides and neoglycoproteins have been obtained by this method in preparative amounts [12], In combination with acid-labile polymeric benzyl ester anchors, this Fmoc technique was applied to solid-phase syntheses of glycopeptides [11,13,14]. 

---