Oligosaccharide industrial applications

Because MBR and CMR enable rapid heating and cooling of reactions, in principle they could be usefully applied to the preparation of kinetic products. A successful example with important industrial applicability is the acid-catalyzed hydrolysis of cellulose to afford practical conversions to glucose and oligosaccharides [12, 15]. Also, treatment of (S)-(+)-carvone in water for 10 min at temperatures between 180 and 250 °C, afforded 8-hydroxy-p-6-menthen-2-one as an intermediate on the pathway to carvacrol [31]. Addition of water to the 8,9 olefinic bond of carvone was regioselective and proceeded at a lower temperature than did isoaromatization (Scheme 3.11). The kinetic product was readily isolated by differential extraction from the recyclable starting ketone, affording a preparative route despite the low conversion. 

A product manufactured in Japan as Alo mixture (Anomalously Linked Oligosaccharides) contains mainly a range of IMOS (glucose, isomaltose, and panose as principal constituents). It is produced from starch as substrate by the action of alpha-amylase, beta-amylase, and a transglucosidase. It is claimed to have favorable properties for application in the food industry.143... 

One of the applications of CE for carbohydrate analysis that has shown increase acceptance in the biotechnology industry is the relative distribution of the N-linked oligossacharides on the conserved asparagines residue on the Fc portion of rMAbs. The advantages of CE over traditional methods include enhanced separation efficiencies, shorter analysis time, and improved robustness. The oligosaccharides present on a monoclonal antibody are asialo-N-linked complex biantennary structures with a core fucose, typical of the structures produced in the Chinese hamster ovary (CHO) cells. The main forms share the fucosylated branched core structure but vary in their terminal galactose occupancy ... 

Future research. From an industrial standpoint, the major requirements of p-mannanases are thermostability and the ability to hydrolyse crystalline and/or amorphous mannan to low DP oligosaccharides. Enzymes with such properties could find application in such processes as coffee extraction and in enzymic treatment of wood pulp. 

A very interesting field in membrane bioreactors is the production of cyclodextrins or oligosaccharides. In general, they have applications in food pharmaceutical, cosmetic agricultural, and plastics industries as emulsifiers, antioxidant, and stabilizing agents. In the food industry cyclodextrins are employed for the preparation of cholesterol-free products. The use of enzymatic membrane reactors to produce... 

Nakano, H., and Kitahata, S. 2005. Application of cyclodextrin glucanotransferases to the synthesis of oligosaccharides and glycosides. In Handbook of Industrial Biocatalysis, ed. C.T. Hon. Boca Raton, FL CRC Press, Taylor Francis Group, pp. 22-1-15. 

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