Oligosaccharide expression

Lundstrom, S.L., Twelkmeyer, B., Sagemark, M.K., Li, 1., Richards, J.C., Hood, D.W., Moxon, E.R., Schweda, E.K.H. Novel globoside-like oligosaccharide expression patterns in nontypeable Haemophilus influenzae lipopolysaccharide. FEBS 1274 (2007) 4886 1903. 

Kobata, A., et al. (1987). BioGel P-4 Column Chromatography of Oligosaccharides Effective Size of Oligosaccharides Expressed in Glucose Units, Methods Enzymol. 138 84-94. 

Transglycosylation. Ea2ymatic transglycosylatioas allow preparatioa of various oligosaccharides that caimot be made readily by stricdy chemical means. The reaction can be expressed as... 

Bryant The work published on the mammalian insulin receptor shows that glycosylation is required for function. There is a mutagenesis study showing that there is a particular Asp residue, without which the receptor is expressed on the membrane but doesn t function (Leconte et al 1994). In other studies the biosynthesis of the oligosaccharides has been inhibited, and this has the same kind of effect (Podskalny et al 1984). 

Caryophyllidae are an interesting source of oligosaccharides and peptides with potential anti-inflammatory and/or immunomodulating effect. These polar compounds might for instance explain the fact that the fresh juice expressed from Aerva lanata (L.) Juss. (Amaranthaceae) inhibits carrageenan-induced edema in rodent. Note that the seeds of Gomphrena species inhibit the formation of IL-6 by osteoblastic cells (MC3T3-E10) without cytotoxicity in vitro. Such property could be useful for the treatment of chronic rheumatoid arthritis, infection, and cancer. In the Lauraceae, trans-cinnamal-dehyde from Cinnamomum cassia (Lauraceae, order Laurales) inhibits in vitro the... 

Fig. 18.—C-l Regions of 13C-N.m.r. Spectra of a,)3-D-Mannopyranans from Pichia pastoris (A) and Citeromyces matritensis (B), and Those of Oligosaccharides Formed by Partial Acetolysis (C and D, Respectively). (Solvent, DjO temperature, 70° chemical shifts expressed as 8C, relative to external tetramethylsilane.)...

Although CE separations can be reasonable well described by the classical theoretical relationships for electrophoretic migration, slight deviations from the theory occur in the case of many classes of solutes. Thus, it has been reported that the CE separation of oligosaccharides follow the general rule [124], while the description of the separation of DNA in polymer solutions necessitated a new mathematical model. The drag forces were expressed by... 

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