Oligonucleotides chimeric

The presence of the chimeric construct in each ars-transformant was verified by PCR using an upstream oligonucleotide that hybridizes to the hydA promoter fragment and a downstream oligonucleotide that hybridizes to the ars gene asindicated with black arrows at the bottom. 

Beetham, P.R. Kipp, P.B. Sawycky, X.L. Amtzen, C.J. May, G.D. (1999) A tool for functional plant genomics chimeric RNA/DNA oligonucleotides cause in vivo gene-specific mutations. Proc. Natl. Acad. Sci. Usa, 96, 8774-8. 

Gamper, Jr., H.B., Cole-Strauss, A., Metz, R., Parekh, H., Kumar, R. and Kmiec, E.B. (2000) A plausible mechanism for gene correction by chimeric oligonucleotides. Biochemistry, 39, 5808-5816. 

Custom synthetic chimeric 2/-0-methyl RNA/DNA oligonucleotides, Integrated DNA Technologies, Inc. 

RNA Set up the annealing reaction with 100 pg ofRNA from the previous transcription reaction. Add to this a fivefold molar excess of your custom synthetic chimeric 2/-0-methyl RNA/DNA oligonucleotides. Bring up to a total volume of250 fil with nuclease-free H20. Denature the reaction for 4 min at 90 °C. Incubate for 10 min at 37 °C to allow the chimeric oligonucleotides to anneal to the RNA. 

Free-solution (gel-free) CE separation has been used in the detection of a target gene sequence amplified using the CPT reaction (see Chapter 9, section 9.2.1.7 for details). The intact chimeric oligonucleotide probe (fluorescein-labeled at the 5 end and biotin-labeled at the 3 end) was separated from a cleaved probe (only fluorescein-labeled at the 5 end). Owing to the presence of biotin in the intact probe, it migrated later than the cleaved probe, even in the absence of a gel sieving matrix [606]. 

There are two reports describing the use of nucleosides with other sugar conformations. A method for preparing internally P-labelled l-DNA has been described using T4 polynucleotide kinase. Chimeric DNA composed of tandem a- and p-anomeric strands have been used in TFOs and shown to have enhanced thermal stability compared to all a- or p-anomeric oligonucleotides. ... 

DNA and DNA/RNA chimeric oligonucleotides were synthesized with a Model 392 DNA/RNA synthesizer from Applied Biosystems (Foster City, CA) with reagents from Glen Research (Sterling, VA). OUgonucleotides were purified by elution from an acrylamide gel after eleetrophoresis. 

To probe for one-dimensional diffusion, we synthesized DNA/RNA chimeric oligonucleotides. Special precautions were taken to avoid ribonuclease contamination during synthesis, purification, and use of these chimeras. For example, all water was treated with diethylpyrocarbonate before exposure to the chimeras. Ribonucleotide 2 -hydroxyl groups were deprotected with 1 M tetrabutyl ammonium fluoride in dimethyl formamide (Aldrich Chemical Milwaukee, WI). Purified oligonucleotides were labeled on the 5 end with [y-32p]ATP (duPont Wilmington, DE) by T4 kinase (Promega Madison, WI), and desalted with a Nick gel filtration column (Pharmacia Uppsala, Sweden). 

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