Synthetic oligonucleotides probe

Figure 1.57 Base-pairing can occur between complementary bases in opposing oligonucleotide strands. These predictable interactions form the basis for using synthetic oligonucleotide probes to target particular DNA sequences.

Cubie, H.A., and Norval, M. (1989) Detection of human papilloma viruses in paraffin wax sections with biotinylated synthetic oligonucleotide probes and immunogold staining./. Clin. Pathol. 42, 988-991. 

Kumar, A., Tchen, P., Roullet, F., and Cohen, J. (1988) Nonradioactive labeling of synthetic oligonucleotide probes with terminal deoxynucleotidyl transferase. Anal. Biochem. 169, 376-382. 

Griffiths, S.L., J.T. Knowler, and M.D. Houslay. 1990. Diabetes-induced changes in guanine-nucleotide-regulatory-protein mRNA detected using synthetic oligonucleotide probes. Eur. J. Biochem. 193 367-374. 

Morris BJ, Haarmann I, Kempter B, Hollt V, Herz A (1986) Localization of prodynorphin messenger RNA in rat brain by in situ hybridization using a synthetic oligonucleotide probe. Neurosci Letts 69 104-108. 

Antibodies can also be used as probes to screen expression libraries, and this strategy has been used successfully in the cloning of vertebrate neuropeptides (17). It is likely that this technique will be helpful in cases in which structural data is particularly difficult to obtain and a variety of highly specific antibodies is available. While these and other techniques will prove valuable in special circumstances, screening with synthetic oligonucleotide probes based on protein data is likely to be the principal method of identifying other peptide precursors in the near future. 

SB Synthetic oligonucleotide probes SSb PCR-generated probes Cloned probes... 

Guitteny, A. F, Fouque, B., Mongin, C., Teoule, R., and Boch, B. (1988) Histological detection of mRNA with biotinylated synthetic oligonucleotide probes. J. Histochem Cytochem. 36, 563-571. 

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