Oleosin genes

Lee, K. and Huang A.H.C. (1991) Genomic nucleotide sequence of a Brassica napus 20-kilodalton oleosin gene. Plant Physiol. 96, 1395-1397. 

Lee, W.S., Tzen, J.T.C., Kridl, J.C., Radke, S.E. and Huang, A.H.C. (1991) Maize oleosin is correctly targeted to seed oil bodies in Brassica napus transformed with the maize oleosin gene. Proc. Natl. Acad. Sci. U.S.A. 88, 6181-6185. 

Chaudhary, S., 1975. Molecular Biology of Flax Unum usitatissimum) Seed Oleosin Genes (Ph.D. thesis). University of Calgary, Calgary, Alberta, pp. 1-290. 

Cao, H., Zhang, L., Tan, X., Long, H., Shockey, J.M., 2014. Identification, classification and differential expression of oleosin genes in tung tree (Vernicia fordii). PLoS One 9, e88409. 

Proteins were then dissolved in 20 mL of 0.125 M Tris-HCl, pH 7.4 containing 1% (m/v) CHAPS and the protease inhibitor cocktail, and treated with CPLL. With several variations from the standard treatments such as other complementary extractions from seeds, the use of urea-containing extraction buffer, and an additional peptide ligand library, the total number of detected gene products was quite large. Two hundred and thirty one unique proteins were found in the pulp (vs. 56 described previously) and 61 in the seeds (vs. only four reported by the literature). In the latter case, the presence of seed storage proteins, oleosins, and histones were detected in the pulp, a thaumatin-like protein, an allergenic protein also named Ole el3, was confirmed. 

Cummins, I., et al. (1993) Differential, temporal and spatial expression of genes involved in storage oil and oleosin accumulation in developing rapeseed embryos. Plant Mol. Biol. 23. 1015-1027. 

Figure 2. An unrooted phylogenetic tree of oleosins. The oleosin numbers after the names of plants follow roughly the chronological appearance of their genes in the GCG data bank. The large and small shaded ovals denote the two seed oleosin isoforms. The large and small shaded rectangles represent the tapetum and pollen (male gametophytic) oleosins, respectively. The shaded circle indicates the female gametophytic oleosin.

Robert, L.S., Gerster, J., Allard, S., Cass, L., and Simmonds, J. (1994) Molecular characterization of two Brassica napus genes related to oleosins which are highly expressed in the tapetum. Plant J. 6, 927-933. 

Lee, K., and Huang, A.H.C. (1994) Genes encoding oleosins in maize kernels of inbreds Mol7 and B73, Plant Mol Biol, 26,1981-1987. 

Very recently, a distinct but similar class of oleosin-like proteins also containing the characteristic central hydrophobic domain has been found in the pollen of rapeseed and corresponding genes have been isolated from both rapeseed and Arabidopsis [1]. Like seeds, pollen cells are propagules which carry their own storage compounds which are required to support their metabolism after germination. In the case of rapeseed and Arabidopsis, Ae seeds and pollen each contain oil bodies and are desiccation tolerant [Ij. This established a correlation between desiccation tolerance and the presence of oil body proteins in both gametophytic and sporophytic plant tissues. 

Tung also contains several genes that encode oleosins. These small strac-tural proteins adopt an unusual thumbtack structure, which allows for the... 

Chen, Y., Zhou, G., Wang, Y, Xu, L., 2010a. F-BOX and oleosin additional target genes for future metabolic engineering in tung trees Ind. Crops. Prod. 32, 684-686. 

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