Oleic acid colonies

An expression construct consisting of the open reading frame of the TnFB A9Ds cDNA inserted into the yeast desaturase expression vector YEpOLEX was used to transform the olel strain of S. cerevisiae as described above. Many transformant colonies were obtained on medium lacking unsaturated fatty acids, indicating complementation of the olel mutation by the encoded T. ni desaturase. GC/MS analysis of the fatty acid methyl esters obtained from the transformants showed that the TnFBA9Ds cDNA encoded a A9 desaturase that produced oleic acid (Z9-18 Acid) and palmitoleic acid (Z9-16 Acid) (Liu et al., 1999). Quantitation of these unsaturated fatty acids under standard conditions as described above revealed about three times more of the former than the latter (Rosenfield et al., 2001). 

On the other hand, oleic acid (0.1 or 0.2 mL per mouse) did not inhibit tumor growth in the spleen Table (7) . Carp oil (0.2 mL per mouse), and oleic acid (0.1 or 0.2 mLper mouse), significantly reduced the number of tumor cell colonies that metastasized in the liver compared with the number in intrasplenic LLC-bearing mice, as shown in Fig. (10) and Table (7) . The growth of the metastatic tumors in the livers of LLC-bearing mice was also inhibited by orally administered carp oil or oleic acid at 0.1 or 0.2 mLper mouse Fig. (11) . 

Table 7. Effects of oleic acid on tbe spleen weight and number of metastatic tumor colonies in the livers of mice with intrasplenically implanted LLC1...

Central America, and in the Cape Verde Isles, the Comores Isles and in some of the former Portuguese colonies, Siam and the East Indies. The percentage fatty acids as triglycerides are oleic, 62% lino-leic, 19%, and saturated fatty acid, the remainder. 

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