Octanol-water purification

Yonezawa, Y. and Y. Urushigawa. 1979. Chemicobiological interactions in biological purification system. V. Relation between biodegradation rate constants of aliphatic alcohols by activated sludge and their partition coefficients in 1-octanol-water system. Chemosphere 3 139-142. 

Chemicals. Purification of n-decane, TMAE, and 1-octanol has been described (2, 3, 8). Oxygen was passed through Drierite to remove water vapor. 

Chemicals and Standard Solutions. Cyclohexanone, cyclohexanol, 1,3,5-trichlorobenzene, 1,2,4-trichlorobenzene, phenol, 4-methylphenol, 4-chloro-phenol, 1,2,3,4-tetrahydroisoquinoline, 1-chlorohexane, 1-chlorododecane, and 1-chlorooctadecane were obtained from Aldrich. Acetone, tetrahydrofuran, ethyl acetate, toluene, dimethyl sulfoxide, and methanol were obtained from J. T. Baker. Distilled-in-glass isooctane, methylene chloride, ethyl ether, and pentane were obtained from Burdick and Jackson. Analytical standard kits from Analabs provided methyl ethyl ketone, isopropyl alcohol, ethanol, methyl isobutyl ketone, tetrachloroethylene, dodecane, dimethylformamide, 1,2-dichlorobenzene, 1-octanol, nitrobenzene, 2,4-dichlorophenol, and 2,5-dichlorophenol. All chemicals obtained from the vendors were of the highest purity available and were used without further purification. High-purity water... 

Chemicals. The 1-octanol used in the partitioning experiments was high purity Eastman White Label reagent ( 871, lot 691-1) and was optically transparent at all wavelengths 220 nm. Additional purification of the octanol via distillation was not necessary. Immediately before partitioning experiments, however, octanol samples were washed with NaOH and water as described below. Deionized, distilled water was used where necessary, and Mallinckrodt analytical reagent perchloric acid ( 2766) was used to prepare the pH 1.0 aqueous phases for partitioning studies (see below). 

A fluorodensitometric assay was developed by Montague and co-workers 140) to analyze cultures from Ochrosia elliptica for ellipticine (1), 9-methoxy-ellipticine (2), and 9-hydroxyellipticine (3) by thin-layer chromatography (TLC) without the need for prior purification. Using silica gel impregnated with dimethyl sulfoxide and a mobile phase of EtOAc-water-l-octanol (17 2 2), these workers were able to achieve good separation of these alkaloids and to assay the resulting chromatograms by fluorodensitometry (40-300 fmol detection limits of alkaloid). 

Benzylpenicillin is an older antibiotic effective against pneumococcal and meningoccal infections, anthrax, and Lyme disease. As part of a purification process. 200 mg of benzyipenicillin is mixed with 25 mL of n-octmol and 25 mL of water. After equilibrium is established, there is a water-rich phase that contains essentially no /t-octanol and ati octanol-rich phase that contains 74. mol % n-octanol and 26 mol % water. Determine the concentrations of benzyipenicillin in each of these phases. 

The chemicals propionic acid (99.9%) and 1-octanol (99.9%) were obtained from Merck and were used without further purification. The purity of these materials were checked by gas chromatography. Distilled water was prepared in our laboratoiy and used throughout all experiments. 

Sodium dodecylsulfate (NaDDS) was supplied by BDH, specially pure grade. Dodecylammonium chloride (DAQ and dodecyltrimethylammonium bromide (DTAB) were supplied by Eastman Kodak. The alcohols 1-hexanol (QOH), 1-heptanol (C7OH), and 1-octanol (CjOH) were supplied by Fluka at their best quality. All compounds were used without further purification. Water was distilled twice immediately before use. All solutions were made by weight. 

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