Reagents perchloric acid

Dipping solution Add 50 ml perchloric acid (70%) carefully to 50 ml water and after cooling dilute the mixture with 50 ml ethanol. 

Spray solution I For steroids 20% aqueous perchloric acid. 

Spray solution II For bile acids 60% aqueous perchloric acid. 

Storage The reagent solutions may be stored for an extended period of 

The chromatogram is freed from solvent, dipped in the reagent solution for 5 — 10 s and then heated to 120—150°C for 5 — 10 min. (Caution Remove perchloric acid from the back of the chromatographic plate ). 

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Ironfim Chloride - Reaction Iron(III) Chloride — Perchloric Acid Reagent 315... 

In particular, sulphuric acid, molybdophosphoric acid, antimony(III)-chloride and fluorescence indicators and others quoted in Table 58 can be used for detection. Richter [142] has obtained blue colours with Zl - and zl -3j8-hydroxysterols in amounts down to 0.03 fig, using the naphthoquinonesulphonic acid-perchloric acid reagent. Reference may be made to Boliiger s chapter (K) for the vitamin D group to section XI on bile acids for bile alcohols (neutral C27-sterols) and to Mangohd s chapter (M) for separation of lipids, including cholesterol and its esters. 

Detection was by spra5dng the chromatograms with ammonium molybdate-perchloric acid reagent (No. 166). 

The amount of phosphorus in the perchloric acid reagent is very small and... 

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