O-Glycerophosphate

Disodium-O-glycerophosphate [819-83-0 (4H2O)] M 216.0, m 102-104 , pKj 6.66 (free acid). Crystd from water. 

O -glycerophosphate derived in situ from glucose. The glucose can also be converted into fatty acids from acetyl-CoA generated via glycolysis and the pyruvate dehydrogenase reaction and from NADPH obtained via the pentose phosphate pathway. 

The above considerations lead to the conclusion that the liver should be capable of synthesizing plasma phospholipides in toto from its various components. This appears to be the case, for a purified enzyme preparar-tion of rat liver has been shown to form diacylphosphatidic acids from fatty acids and L-a-glycerophosphate. i The diacylphosphatidic acids need only a nitrogenous base in ester linkage with the phosphate to form lecithin (or a cephalin). The following mechanism was postulated for the synthesis of distearylphosphatidic acid from stearic acid-l-C and L-o-glycerophosphate-P (a-G-P) by the rat liver preparation ... 

Fig. 1. Prostatic acid phosphatase activity as a function of pH ( ) phenyl phosphate (O) p-nitrophenyl phosphate and (A) /8-glycerophosphate. Buffers Ac, acetate Cit, citrate and tris. From Nigam et al. (88).

Fiq. 36. Absorption spectrum of partially purified -glycerophosphate dehydroge-nase, protein concentration 13 mg/ml. (O) Spectrum of oxidized enzyme. The difference spectra (shown in the insert) were recorded after the addition of a few granules of sodium hydrosulfite ( ) or 5 Mmoles substrate (O) in n total volume of 0.2 ml. In the difference spectra, a decrease in optical density indicates bleaching. From Ringler (115). 

B21. Bodansky, O., The energy of activation of the hydrolysis of sodium /3-glycerophosphate by bone phosphatase at optimal pH. J, Biol. Chem. 129, 197-206 (1939). 

Sargent JD, Dalton MA, O Connor GT, Olmstead EM, Klein RZ. Randomized trial of calcium glycerophosphate-supplemented infant formula to prevent lead absorption. Am J Clin Nutr 1999 69(6) 1224-30. 

Schwartz, M. K., Kessler, G., and Bodansky, O., Comparison of serum alkaline phosphatase activities determined with sodium beta glycerophosphate and sodium phenylphosphate as substrates. Am. J. Clin. Pathol. 275-280 (1960). 

Glycerophosphate hexahydrate, < 4-ti4jN4Ol0P.6H,O. one gram dissolves in about 350 ml water, about 310 ml alcohol. Slightly sol in chloroform very slightly in ether. 

Other chromogenic substrates can also be exploited, including phenolphthalein monophosphate, thymophthalein monophosphate, P glycerophosphate, and uridine phosphate. Fluorigenic substrates can also be used such as P-naphthyl phosphate, 4-methylumbelliferyl phosphate, and 3-o-methylfluorescein monophosphate (21). 

Bis (4-(1,1-dimethylethyl) benzoato-o) hydroxy aluminum Cadmium stearate Calcium glycerophosphate Calcium phosphate dibasic Calcium phosphate monobasic anhydrous... 

The acylation of dihydroxyacetone phosphate and the subsequent reduction of acyldihydroxacetone phosphate to monoacylphosphatidic acid provides an alternative route for phosphatidic acid synthesis. Acyltransferases which utilize dihydroxyacetone phosphate have been purified from several sources (Bell and Coleman, 1982). However, the relative contributions of dihydroxyacetone phosphate and glycerophosphate to phosphatidic acid synthesis remain controversial (O Doherty, 1978). 

The acylphosphonyl derivative 127, as an inhibitor of PI-PLC has also been reported. (See also Vol. 27, p. 203, ref 154 for related work). A short synthesis of no inositol 3,4,5-trisphosphate l-glycerophosphate and of D-2-deoxy-3-fluoro (and 3-chloro) - i>o-inoffltoi 1-glycerophosphate derivatives have also been reported. 

The acid yeast phosphatase mentioned above is markedly inhibited by thiamine, and to a lesser degree by the pyrimidine part of the vitamin, not only when TPP is the substrate, but also when other phosphate esters, such as (X- or 8-glycerophosphate, are used. Animal phosphatases do not appear to be inhibited by thiamine o. 

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